Association between endothelial nitric oxide synthase and the renin-angiotensin-aldosterone system polymorphisms, blood pressure and training status in normotensive/pre-hypertension and hypertensive older adults: a pilot study

Autor(es):Mostrar menos -

da Silva, Roberta Fernanda ^[1] ; Lacchini, Riccardo ^[2] ; Pinheiro, Lucas Cezar ^[2] ; Ferezin, Leticia Perticarrara ^[3] ; Tanus-Santos, Jose Eduardo ^[4] ; Luizon, Marcelo Rizzatti ^[5] ; Dionisio, Thiago Jose ^[6] ; Santos, Carlos Ferreira ^[6] ; Reia, Thais Amanda ^[1] ; Jacomini, Andre Mourao ^[1] ; Moreno, Ana Maria Guilmo ^[1] ; Zago, Anderson Saranz ^{[1, 7]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Sci, Postgrad Program Movement Sci, Dept Phys Educ, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Dept Psychiat Nursing & Human Sci, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao, Dept Pharmacol, Ribeirao Preto, SP - Brazil

^[5] Univ Minas Gerais Fed, Inst Biol Sci, Dept Genet Ecol & Evolut, Belo Horizonte, MG - Brazil

^[6] Univ Sao Paulo, Bauru Sch Dent, Dept Pharmacol, Bauru, SP - Brazil

^[7] Sao Paulo State Univ Unesp, Sch Sci, Ctr Noncommun Dis Aging & Exercise Stud CEDEE, Dept Phys Educ, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL AND EXPERIMENTAL HYPERTENSION; v. 43, n. 7, p. 661-670, OCT 3 2021.
• Citações Web of Science: 0

Resumo

Introduction:Variations in blood pressure (BP) are, in part, genetically determined and some polymorphisms of renin-angiotensin- aldosterone system (RAAS) and synthase of endothelial nitric oxide (eNOS) have been related to hypertension (HT). Conversely, physical exercise is considered a non-pharmacological tool for HT control, treatment, and prevention. Objective: The purpose of this study is to investigate the relationship between eNOS and RAAS polymorphisms, their epistatic interaction, and the respective humoral factors in the BP control in normotensive/pre-hypertension and hypertensive older adults and how this relationship can be modulated by training status (TS) level. Methods:A total of 155 older adults (66.94 +/- 6.83 years old) performed the following evaluations: AAHPERD battery test to determine the general functional fitness index (GFFI), systolic and diastolic blood pressure (SBP and DBP), blood collection for DNA extraction, analysis of eNOS gene polymorphisms rs2070744; rs61722009 and rs1799983 and RAAS polymorphisms rs699; rs1799752 and rs5186, and quantification of ACE activity (Fluorimetric Assay) and nitrite concentration (Chemiluminescence Method). Results and Conclusion:Good TS level appears to exert greater influence on SBP for G2 and G3 (G1: 125.79 +/- 14.03/ G2: 119.91 +/- 11.72(/)G3: 119.71 +/- 10.85) and on NO2 for G3 (G1: 0.42 +/- 0.25/ G2: 0.54 +/- 0.45/ G3: 0.71 +/- 0.52). No associations were observed between eNOS and RAAS polymorphisms, but the epistasis was identified between eNOS polymorphism, rs2070744, and RAAS polymorphism, rs699, revealing a statistically significant interaction (p = .0235) with training score of 0.63, a training test accuracy of 0.61 and a cross-validation consistency of 10/10. This result suggests an increased risk of hypertension. (AU)

• Processo FAPESP: 15/24847-8 - Variação dos mecanismos de regulação da pressão arterial durante a realização do exercício físico agudo em adultos e idosos, hipertensos e normotensos e com diferentes níveis de condicionamento físico
• Beneficiário: Anderson Saranz Zago
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Temático