Characterization of murine extracellular vesicles and Toxoplasma gondii infection

Autor(es)

Maia, Marta Marques ^[1] ; da Cruz, Allecineia Bispo ^[1] ; Pereira, Ingrid de Siqueira ^[1] ; Taniwaki, Noemi Nosomi ^[2] ; Namiyama, Gislene Mitsue ^[2] ; Pereira-Chioccola, Vera Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Nucleo Microscopia Eletron, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 43, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

This study characterized extracellular vesicles (EVs) of sera from mice infected with Toxoplasma gondii or immunized with EVs derived T gondii. EVs were purified of sera from four groups (5 A/Sn mice/group). EV-IM: Mice immunized with T gondii-released EVs; ACT: mice in acute infection; CHR: mice in chronic infection; and NI: normal mice. EVs were purified by ultracentrifugation. Concentration of serum-derived EVs from NI group was smaller than EV-IM, ACT and CHR groups. Most of the EVs from ACT and CHR groups were microvesicles, and they were bigger than the NI group. The same results were shown by Transmission Electron Microscopy. The presence of exosomes was shown in immunoblotting by tetraspanin (CD63 and CD9) evidence. Splenocytes of EV-IM, CHR and NI groups were stimulated with T. gondii derived EVs. EV-IM and CHR groups up-expressed IFN-gamma; TNF-alpha and IL-17, when compared with the NI group. IL-10 was up-expressed only in the EV-IM group. EV-IM, ACT and CHR groups expressed more miR-155-5p, miR-29c-3p and miR-125b-5p than the NI group. Host-T gondii interaction can occur, also, via EVs. miRNAs participate in the modulation of cellular immune response against T gondii. These data give subsidies to propose the differentiation between infect or noninfect hosts by concentration of EVs. (AU)

• Processo FAPESP: 18/04709-8 - Estudo das vesículas extracelulares de Toxoplasma gondii modulando o sistema imune de hospedeiros infectados
• Beneficiário: Vera Lúcia Pereira Chioccola
• Modalidade de apoio: Auxílio à Pesquisa - Regular