Determination of sulfate in algal polysaccharide samples: a step-by-step protocol using microplate reader

Autor(es)

Torres, Priscila Bezerra ^[1] ; Nagai, Alice ^[1] ; Palacios Jara, Carmen Eusebia ^[1] ; Santos, Janaina Pires ^[1] ; Chow, Fungyi ^[1] ; Alves Cursino dos Santos, Deborah Yara ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, A101, Tv 14, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Ocean and Coastal Research; v. 69, 2021.
• Citações Web of Science: 0

Resumo

Abstract Algal polysaccharides exhibit a wide range of biological activities and potential applications. Many of these bioactivities correlate positively with the presence of sulfate groups on the polysaccharides. The most common method used for sulfate quantification in algal samples is the turbidimetric method using the barium chloride-gelatin reagent. However, the original procedure is difficult to adapt for routine analysis since it is laborious and time-consuming. An optimized method was established using 96-well microplates, with the advantage of reducing waste and discrimination between organic and inorganic sulfates. This proposed method produced the same accuracy as the original. (AU)

• Processo FAPESP: 13/07543-0 - Atividades pesticida de acetogeninas de espécies de Annonaceae nativas do Cerrado
• Beneficiário: Déborah Yara Alves Cursino dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/50731-1 - Biodiversidade fisiológica e química de macroalgas ecológica e economicamente importantes
• Beneficiário: Fanly Fungyi Chow Ho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular