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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of sulfate in algal polysaccharide samples: a step-by-step protocol using microplate reader

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Torres, Priscila Bezerra [1] ; Nagai, Alice [1] ; Palacios Jara, Carmen Eusebia [1] ; Santos, Janaina Pires [1] ; Chow, Fungyi [1] ; Alves Cursino dos Santos, Deborah Yara [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, A101, Tv 14, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Ocean and Coastal Research; v. 69, 2021.
Web of Science Citations: 0

Algal polysaccharides exhibit a wide range of biological activities and potential applications. Many of these bioactivities correlate positively with the presence of sulfate groups on the polysaccharides. The most common method used for sulfate quantification in algal samples is the turbidimetric method using the barium chloride-gelatin reagent. However, the original procedure is difficult to adapt for routine analysis since it is laborious and time-consuming. An optimized method was established using 96-well microplates, with the advantage of reducing waste and discrimination between organic and inorganic sulfates. This proposed method produced the same accuracy as the original. (AU)

FAPESP's process: 13/50731-1 - Physiological and chemical biodiversity of macroalgae ecologically and economically important
Grantee:Fanly Fungyi Chow Ho
Support type: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 13/07543-0 - Pesticide activities of acetogenins of native species of Annonaceae from Brazilian Savanna
Grantee:Déborah Yara Alves Cursino dos Santos
Support type: Regular Research Grants