Effect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study

Autor(es)

Freitas da Silva, Emily Vivianne ^[1] ; Goiato, Marcelo Coelho ^{[2, 3]} ; Bitencourt, Sandro Basso ^[1] ; Finer, Yoav ^{[4, 5]} ; Balera Brito, Victor Gustavo ^[5] ; Takamiya, Aline Satie ^{[3, 5]} ; Penha de Oliveira, Sandra Helena ^{[3, 6]} ; Micheline dos Santos, Daniela ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, UNESP, Jose Bonifacio St 1153, BR-16015050 Aracatuba, SP - Brazil

^[2] UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ, Oral Oncol Ctr, UNESP, Sch Dent, 1193 Jose Bonifacio St, BR-15050015 Aracatuba, SP - Brazil

^[4] Univ Toronto, Fac Dent, Toronto, ON - Canada

^[5] Univ Toronto, Inst Biomed Engn, Toronto, ON - Canada

^[6] UNESP, Dept Basic Sci, Aracatuba Dent Sch, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 76, OCT 2021.
• Citações Web of Science: 0

Resumo

The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 degrees C or 100 degrees C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min +/- 60 degrees C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p < 0.05). WB with longer processing time at higher temperature had highest DC (85.6%) and higher TGF beta 1-gene expression (1.39); long cycle low power MW showed lowest DC (69.6%), lower cell proliferation (85.4%, MTT), and large IL-2 release (39,297 ng/mL). AP with additional processing time showed lower cell proliferation (75.3%, Alamar Blue), and AP polymerized at room temperature showed higher CASP 9-gene expression (1.21). AP methods showed higher IL-6 release (>277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity. (AU)

• Processo FAPESP: 15/20221-7 - Biocompatibilidade da resina acrílica de prótese ocular: análise in vitro e in vivo
• Beneficiário: Emily Vivianne Freitas da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/03455-7 - Biocompatibilidade da resina acrílica de prótese ocular: análise in vitro e in vivo
• Beneficiário: Daniela Micheline dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular