| Full text | |
| Author(s): |
Freitas da Silva, Emily Vivianne
[1]
;
Goiato, Marcelo Coelho
[2, 3]
;
Bitencourt, Sandro Basso
[1]
;
Finer, Yoav
[4, 5]
;
Balera Brito, Victor Gustavo
[5]
;
Takamiya, Aline Satie
[3, 5]
;
Penha de Oliveira, Sandra Helena
[3, 6]
;
Micheline dos Santos, Daniela
[2, 3]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, UNESP, Jose Bonifacio St 1153, BR-16015050 Aracatuba, SP - Brazil
[2] UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP - Brazil
[3] Sao Paulo State Univ, Oral Oncol Ctr, UNESP, Sch Dent, 1193 Jose Bonifacio St, BR-15050015 Aracatuba, SP - Brazil
[4] Univ Toronto, Fac Dent, Toronto, ON - Canada
[5] Univ Toronto, Inst Biomed Engn, Toronto, ON - Canada
[6] UNESP, Dept Basic Sci, Aracatuba Dent Sch, Aracatuba, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | TOXICOLOGY IN VITRO; v. 76, OCT 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 degrees C or 100 degrees C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min +/- 60 degrees C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p < 0.05). WB with longer processing time at higher temperature had highest DC (85.6%) and higher TGF beta 1-gene expression (1.39); long cycle low power MW showed lowest DC (69.6%), lower cell proliferation (85.4%, MTT), and large IL-2 release (39,297 ng/mL). AP with additional processing time showed lower cell proliferation (75.3%, Alamar Blue), and AP polymerized at room temperature showed higher CASP 9-gene expression (1.21). AP methods showed higher IL-6 release (>277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity. (AU) | |
| FAPESP's process: | 15/20221-7 - Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis |
| Grantee: | Emily Vivianne Freitas da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/03455-7 - Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis |
| Grantee: | Daniela Micheline dos Santos |
| Support Opportunities: | Regular Research Grants |