Rubia Gallega x Nelore crossbred cattle improve beef tenderness through changes in protein abundance and gene expression

Autor(es):Mostrar menos -

Ferrinho, Adrielle M. ^[1] ; de Moura, Gabriella Vespe ^[1] ; Martins, Taiane da Silva ^[2] ; Munoz, Julian ^[1] ; Mueller, Lenise F. ^[1] ; Garbossa, Pollyana Leite Matioli ^[2] ; de Amorim, Tamyres Rodrigues ^[1] ; Jessica, Luana Gemelli ^[1] ; Fuzikawa, Ingrid Harumi de Souza ^[1] ; Prado, Cibele ^[1] ; da Silveira, Juliano Coelho ^[1] ; Poleti, Mirele D. ^[1] ; Baldi, Fernando ^[3] ; Pereira, Angelica S. C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, USP, Fac Anim Sci & Food Engn, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, USP, BR-13635900 Pirassununga, SP - Brazil

^[3] Sao Paulo State Univ, Fac Agr & Vet Sci, UNESP, BR-14884000 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIVESTOCK SCIENCE; v. 251, SEP 2021.
• Citações Web of Science: 0

Resumo

The objective was to evaluate meat quality of Rubia Gallega x Nelore (RGN) crossbred compared to Nelore (N) cattle, using mRNA analysis of calpain 1 and 2, calpastatin, myostatin, and TGF beta; and protein abundance profile post mortem based on aged beef of the different genetic groups. Sixteen N and 16 RGN were finished in feedlot by 120 days and harvested. RGN beef presented greater tenderness. Transcripts analysis showed increased levels of TGF beta and MSTN in RGN, indicating differences in muscle fiber formation. However, no difference was observed in mRNA for CAPN1, CAPN2 and CAST. Protein analysis demonstrated increased troponin-T degradation in RGN. Interaction for HSP 27 degradation was observed, indicating the degradation of this protein is faster and greater in RGN than N. No difference in myostatin protein abundance was found. In general, RGN cattle improves beef quality and these differences may be explained by TGF beta and MSTN gene expression, troponin-T degradation and HSP abundance. (AU)

• Processo FAPESP: 18/01457-8 - Expressão de genes envolvidos no perfil de ácidos graxos e proteólise post mortem de bovinos Nelore e Rubia Gallega x Nelore
• Beneficiário: Adrielle Matias Ferrinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/50788-0 - Carne bovina e saúde: uma abordagem holística do perfil de ácidos graxos
• Beneficiário: Angélica Simone Cravo Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular