An in silico, structural, and biological analysis of lactoferrin of different mammals

Autor(es)

Vieira, Dielson da S. ^[1] ; Polveiro, Richard C. ^[2] ; Butler, Thomas J. ^[3] ; Hackett, Timothy A. ^[4] ; Braga, Camila P. ^[4] ; Puniya, Bhanwar Lal ^[4] ; Teixeira, Weslen F. P. ^[5] ; Padilha, Pedro de M. ^[6] ; Adamec, Jiri ^[4] ; Feitosa, Francisco L. F. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Julio Mesquita Filho UNESP, Sch Vet Med, Aracatuba, SP - Brazil

^[2] Fed Univ Vicosa UFV, Vet Dept, Vicosa, MG - Brazil

^[3] Technol Univ Dublin, Sch Biol & Hlth Sci, Environm Sustainabil & Hlth Inst ESHI, Dublin 7 - Ireland

^[4] Univ Nebraska Lincoln, Dept Biochem, Lincoln, NE - USA

^[5] Fed Univ Goias UFG, Dept Vet Med, Goiania, Go - Brazil

^[6] Sao Paulo State Univ Julio Mesquita Filho UNESP, Biosci Inst, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 187, p. 119-126, SEP 30 2021.
• Citações Web of Science: 0

Resumo

Lactoferrin (LF) belongs to the family of transferrins having multifunctional roles associated with the immune system of animals. To follow the aims for this study was selected 20 sequences of LF from mammalian species to evaluate the chemical, biological, and structural properties. Bioinformatics approaches used programs such as MAFFT for sequence alignment; PartitionFinder and MrBayes for phylogenetic approaches; I-TASSER, PRO-CHECK, Molecular Operating Environment (MOE), SWISS Model server, Peptide DB and Expasy ProtParam to estimate the physicochemical properties, to model the protein and predicted secondary structures. A phylogenic analysis shows species with genetic similarities clustered by complexity and unique grouping between Capra hircus, Macaca mulatta, and Myotis lucifugus, since they presented more amino acids but not overall changes in the iron-binding sites or biological aspects. Structural deviations in these clusters obtained in LF from those species were found in residues 46 (position 406-450), that is part of alpha-helix, and 37 (position 295-331), that is part of the beta-sheets. Our predicted model can be used to investigate more about structural aspects of LF and be applied for medicinal research. (AU)

• Processo FAPESP: 17/22041-1 - Proteoma da transição do colostro para o leite em cabras: interações protéicas para a maturação dos sistemas imunológico e digestivo do recém-nascido
• Beneficiário: Dielson da Silva Vieira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/02781-6 - Avaliação do perfil de proteínas para o desenvolvimento intestinal e imunológico em cabras e vacas leiteiras
• Beneficiário: Dielson da Silva Vieira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado