Autor(es)

Gruner, Malte C. ^[1] ; Leite, Ilaiali S. ^[2] ; Inada, Natalia M. ^[2] ; de Camargo, Andrea S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, Lab Spect Funct Mat LEMAF, Ave Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Lab Biophoton, Opt Grp, Av Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
• Citações Web of Science: 0

Resumo

In this study a straightforward strategy to deliver photosensitizing molecules into cancer cells by using Laponite RD (R) (LAP) nanodisks as carrier is presented. We report the application of LAP functionalized with a highly hydrophobic Silicon phthalocyanine photosensitizer (SiPc) for efficient cell uptake and photodynamic therapy (PDT) of MCF-7 breast cancer cells in vitro. This inorganic-organic hybrid nanomaterial caused a threefold increase in intracellular ROS levels and 99.7% of cancer cell inactivation after light treatment (630 nm; fluency of 108 J/cm(2)). With its theranostic capabilities (simultaneous fluorescence and singlet oxygen generation), uptake into cancer cells was monitored to control the photo-inactivation. The functionalized nanodisks already proved to be an efficient photo-inactivator of pathogenic Gram-(+) bacteria and were previously reported as LS10. By extending the use of LS10 to PDT of cancer cells, it is an example of a photosensitizer functionalized nanoclay with multipurpose drug characteristics, demonstrating an intriguing potential for future phototherapeutic studies and applications. (AU)

• Processo FAPESP: 13/07793-6 - CeRTEV - Centro de Pesquisa, Tecnologia e Educação em Materiais Vítreos
• Beneficiário: Edgar Dutra Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/24118-6 - Estudo de nanopartículas emissoras de luz (nanóforos conversores NIR-VIS) para aplicações biomédicas e biotecnológicas
• Beneficiário: Malte Christian Gruener
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/54035-4 - Emu: facility para estudos avancados de materiais nanoestruturados e biossistemas/fama
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários