Photosensitizing nanoclays for efficient cell uptake and in vitro photodynamic therapy

Author(s)

Gruner, Malte C. ^[1] ; Leite, Ilaiali S. ^[2] ; Inada, Natalia M. ^[2] ; de Camargo, Andrea S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, Lab Spect Funct Mat LEMAF, Ave Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Lab Biophoton, Opt Grp, Av Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
• Web of Science Citations: 0

Abstract

In this study a straightforward strategy to deliver photosensitizing molecules into cancer cells by using Laponite RD (R) (LAP) nanodisks as carrier is presented. We report the application of LAP functionalized with a highly hydrophobic Silicon phthalocyanine photosensitizer (SiPc) for efficient cell uptake and photodynamic therapy (PDT) of MCF-7 breast cancer cells in vitro. This inorganic-organic hybrid nanomaterial caused a threefold increase in intracellular ROS levels and 99.7% of cancer cell inactivation after light treatment (630 nm; fluency of 108 J/cm(2)). With its theranostic capabilities (simultaneous fluorescence and singlet oxygen generation), uptake into cancer cells was monitored to control the photo-inactivation. The functionalized nanodisks already proved to be an efficient photo-inactivator of pathogenic Gram-(+) bacteria and were previously reported as LS10. By extending the use of LS10 to PDT of cancer cells, it is an example of a photosensitizer functionalized nanoclay with multipurpose drug characteristics, demonstrating an intriguing potential for future phototherapeutic studies and applications. (AU)

• FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
• Grantee: Edgar Dutra Zanotto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 15/24118-6 - Study of light emitting nanoparticles (upconverting nanophores) for biomedical and biotechnological applications
• Grantee: Malte Christian Gruener
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program