Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

Autor(es)

Abdeshahian, Peyman ^[1] ; Ascencio, Jesus Jimenez ^[1] ; Philippini, Rafael R. ^[1] ; Antunes, Felipe Antonio Fernandes ^[1] ; Ingle, Avinash P. ^[2] ; Abdeshahian, Mojgan ^[3] ; dos Santos, Julio Cesar ^[1] ; da Silva, Silvio Silverio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, BR-12602810 Sao Paulo - Brazil

^[2] Panjabrao Deshmukh Krishi Vidyapeeth, Dept Agr Bot, Biotechnol Ctr, Akola 444104 - India

^[3] Payame Noor Univ PNU, Dept Agr Sci, Tehran 193954697 - Iran

• Tipo de documento: Artigo Científico
• Fonte: SUSTAINABILITY; v. 13, n. 17 SEP 2021.
• Citações Web of Science: 0

Resumo

Lasiodiplodan is a beta-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 degrees C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL(-1) of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg(-1) glucose consumed and 0.016 gL(-1) h(-1), respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a beta-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan. (AU)

• Processo FAPESP: 18/14095-7 - Novas estratégias integradas para produção econômica de beta-glucana a partir de resíduos agroindustriais e roteiro para comercialização no Brasil
• Beneficiário: Peyman Abdeshahian
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático