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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

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Author(s):
Abdeshahian, Peyman [1] ; Ascencio, Jesus Jimenez [1] ; Philippini, Rafael R. [1] ; Antunes, Felipe Antonio Fernandes [1] ; Ingle, Avinash P. [2] ; Abdeshahian, Mojgan [3] ; dos Santos, Julio Cesar [1] ; da Silva, Silvio Silverio [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, BR-12602810 Sao Paulo - Brazil
[2] Panjabrao Deshmukh Krishi Vidyapeeth, Dept Agr Bot, Biotechnol Ctr, Akola 444104 - India
[3] Payame Noor Univ PNU, Dept Agr Sci, Tehran 193954697 - Iran
Total Affiliations: 3
Document type: Journal article
Source: SUSTAINABILITY; v. 13, n. 17 SEP 2021.
Web of Science Citations: 0
Abstract

Lasiodiplodan is a beta-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 degrees C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL(-1) of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg(-1) glucose consumed and 0.016 gL(-1) h(-1), respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a beta-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan. (AU)

FAPESP's process: 18/14095-7 - Novel integrated strategies for economic beta-glucan production from agro-industrial residues and road map for commercialization in Brazil
Grantee:Peyman Abdeshahian
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants