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Production of ethanol from bagasse sugarcane pretreated with ozone: study of inhibitors, fermentation and industrial yeasts


This project fits in with the theme of biofuels to contribute with knowledge that support concrete progress in strengthening the renewable energy sources. Hydrolysis of abundant plant biomass in Brazil, such as bagasse and straw of cane sugar, have been widely investigated envisioning their use in biochemical fermentative route. The aim of this project is to evaluate integrally the process of production of second generation ethanol, starting with the bagasse until obtain ethanol. To achieve this goal, the following steps will be performed: (1) pretreatment of bagasse using ozone for delignification and increased permeability in the cellulosic fiber, (2) enzymatic saccharification of biomass using commercial enzymatic formulations and / or own production, (3 ) fermentation using different industrial yeast strains Saccharomyces cerevisiae (Cat1, Pedra2, JP1). With the execution of the fermentative steps, it will be possible to close the circle of the process to the final product and, thus, to obtain information in an integrated way of the three important steps required for the production of second generation ethanol. (AU)

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ORDUNA ORTEGA, JULIETH; MORA VARGAS, JORGE ANDRES; METZKER, GUSTAVO; GOMES, ELENI; DA SILVA, ROBERTO; BOSCOLO, MAURICIO. Enhancing the production of the fermentable sugars from sugarcane straw: A new approach to applying alkaline and ozonolysis pretreatments. RENEWABLE ENERGY, v. 164, p. 502-508, FEB 2021. Web of Science Citations: 1.
DE SOUZA, ANGELICA R.; DE ARAUJO, GABRIELA C.; ZANPHORLIN, LETICIA M.; RULLER, ROBERTO; FRANCO, FERNANDA C.; TORRES, FERNANDO A. G.; MERTENS, JEFFREY A.; BOWMAN, MICHAEL J.; GOMES, ELENI; DA SILVA, ROBERTO. Engineering increased thermostability in the GH-10 endo-1,4-beta-xylanase from Thermoascus aurantiacus CBMAI 756. International Journal of Biological Macromolecules, v. 93, n. A, p. 20-26, DEC 2016. Web of Science Citations: 8.
PERRONE, OLAVO MICALI; COLOMBARI, FELIPPE MARIANO; ROSSI, JESSIKA SOUZA; SOUZA MORETTI, MARCIA MARIA; BORDIGNON, SIDNEI EMILIO; CARREIRA NUNES, CHRISTIANE DA COSTA; GOMES, ELENI; BOSCOLO, MAURICIO; DA-SILVA, ROBERTO. Ozonolysis combined with ultrasound as a pretreatment of sugarcane bagasse: Effect on the enzymatic saccharification and the physical and chemical characteristics of the substrate. Bioresource Technology, v. 218, p. 69-76, OCT 2016. Web of Science Citations: 15.
DE CASSIA PEREIRA, J.; PAGANINI MARQUES, N.; RODRIGUES, A.; BRITO DE OLIVEIRA, T.; BOSCOLO, M.; DA SILVA, R.; GOMES, E.; BOCCHINI MARTINS, D. A. Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification. Journal of Applied Microbiology, v. 118, n. 4, p. 928-939, APR 2015. Web of Science Citations: 29.
PEREIRA, JOSIANI DE CASSIA; RIBEIRO LEITE, RODRIGO SIMOES; ALVES DO PRADO, HELOIZA FERREIRA; BOCCHINI MARTINS, DANIELA ALONSO; GOMES, ELENI; DA SILVA, ROBERTO. Production and Characterization of beta-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31. Applied Biochemistry and Biotechnology, v. 175, n. 2, p. 723-732, JAN 2015. Web of Science Citations: 7.
DE SOUZA MORETTI, MARCIA MARIA; BOCCHINI-MARTINS, DANIELA ALONSO; CARREIRA NUNES, CHRISTIANE DA COSTA; VILLENA, MARIA AREVALO; PERRONE, OLAVO MICALI; DA SILVA, ROBERTO; BOSCOLO, MAURICIO; GOMES, ELENI. Pretreatment of sugarcane bagasse with microwaves irradiation and its effects on the structure and on enzymatic hydrolysis. APPLIED ENERGY, v. 122, p. 189-195, JUN 1 2014. Web of Science Citations: 67.
OLIVEIRA, D. S.; TELIS-ROMERO, J.; DA-SILVA, R.; FRANCO, C. M. L. Effect of a Thermoascus aurantiacus thermostable enzyme cocktail on wheat bread qualitiy. Food Chemistry, v. 143, p. 139-146, JAN 15 2014. Web of Science Citations: 16.
ARAKAKI, R. L.; MONTEIRO, D. A.; BOSCOLO, M.; DASILVA, R.; GOMES, E. Halotolerance, ligninase production and herbicide degradation ability of basidiomycetes strains. Brazilian Journal of Microbiology, v. 44, n. 4, p. 1207-1214, OCT-DEC 2013. Web of Science Citations: 11.

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