Bioactivity effects of extracellular matrix proteins on apical papilla cells

Autor(es)

Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; Anovazzi, Giovana ^[3] ; Wu Mon, Filipe Koon ^[4] ; Bordini, Ester Alves Ferreira ^[1] ; Hebling, Josimeri ^[3] ; De Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Fac Odontol Bauru, Dept Dent Endodontia & Mat Odontol, Bauru, SP - Brazil

^[3] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP - Brazil

^[4] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 29, 2021.
• Citações Web of Science: 0

Resumo

Abstract Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; α=0.05). Results We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 µg/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 µg/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 µg/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion We conclude that FN at 10 µg/mL concentration exerted the most intense bioactive effects on hAPCs. (AU)

• Processo FAPESP: 17/22739-9 - Desenvolvimento e análise de um scaffold bicamada para regeneração do complexo dentino-pulpar
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14210-8 - Síntese e avaliação de biomateriais associados a proteínas da matriz extracelular para a regeneração do tecido pulpar
• Beneficiário: Maria Luísa de Alencar e Silva Leite
• Modalidade de apoio: Bolsas no Brasil - Doutorado