| Full text | |
| Author(s): |
Leite, Maria Luisa
[1]
;
Soares, Diana Gabriela
[2]
;
Anovazzi, Giovana
[3]
;
Wu Mon, Filipe Koon
[4]
;
Bordini, Ester Alves Ferreira
[1]
;
Hebling, Josimeri
[3]
;
De Souza Costa, Carlos Alberto
[4]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Fac Odontol Bauru, Dept Dent Endodontia & Mat Odontol, Bauru, SP - Brazil
[3] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP - Brazil
[4] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of Applied Oral Science; v. 29, 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Abstract Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; α=0.05). Results We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 µg/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 µg/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 µg/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion We conclude that FN at 10 µg/mL concentration exerted the most intense bioactive effects on hAPCs. (AU) | |
| FAPESP's process: | 17/22739-9 - Development and analysis of a bilayer scaffold for pulp-dentin regeneration |
| Grantee: | Carlos Alberto de Souza Costa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/14210-8 - Synthesis and evaluation of biomaterials associated with extracellular matrix proteins for the pulp tissue regeneration |
| Grantee: | Maria Luísa de Alencar e Silva Leite |
| Support Opportunities: | Scholarships in Brazil - Doctorate |