Brazilian sunberry (Solanum oocarpum Sendtn): Alkaloid composition and improvement of mitochondrial functionality and insulin secretion of INS-1E cells

Autor(es)

Aparecida Pereira, Ana Paula ^{[1, 2]} ; Figueiredo Angolini, Celio Fernando ^[3] ; de Souza-Sporkens, Jane Cristina ^[4] ; da Silva, Tomaz Antonio ^[3] ; Franco de Oliveira, Helena Coutinho ^[4] ; Pastore, Glaucia Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Fac Food Engn, Dept Food Sci, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Mato Grosso, Fac Nutr, Dept Food & Nutr, Cuiaba, MG - Brazil

^[3] Univ ABC UFABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

^[4] Univ Estadual Campinas, Biol Inst, Dept Struct & Funct Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 148, OCT 2021.
• Citações Web of Science: 0

Resumo

Chronic high-glucose levels induce the generation of reactive oxygen species leading to mitochondrial dysfunction, which is one of the pathological triggers in the development of diabetes. This study investigated the alkaloid composition of two fruits of the genus Solanum, fruta-do-lobo (Solanum lycocarpum) and ju acute accent a-acu (So-lanum oocarpum), and their capacity to protect against oxidative damage and defective insulin secretion induced by chronic high-glucose levels. LC-MS and molecular network of fruit crude extracts reveals that ju acute accent a-acu and fruta-do-lobo contain kukoamines and glycoalkaloids, respectively. Two purification processes were used to enrich those alkaloids. Fruta-do-lobo extract rich in glycoalkaloids showed a strong cytotoxicity effect, however the ju acute accent a-acu enriched extract was able to protect mitochondrial functionality against glucotoxicity and stimulate insulin secretion even under conditions of hyperglycemia. These results are promising and suggest that ju acute accent a-acu is a potential source of bioactive compounds for adjuvant/co-adjuvant therapy for diabetes. (AU)

• Processo FAPESP: 19/08853-9 - Ecologia química no estudo de interações planta-microrganismos em busca de uma agricultura sustentável
• Beneficiário: Célio Fernando Figueiredo Angolini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/17728-8 - Função e disfunção mitocondrial: implicações para o envelhecimento e doenças associadas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
• Beneficiário: Glaucia Maria Pastore
• Modalidade de apoio: Auxílio à Pesquisa - Temático