Cell-to-cell trafficking patterns in cell lines of Araucariaangustifolia (Brazilian pine) with contrasting embryogenic potential

Autor(es)

Navarro, V, Bruno ; Elbl, Paula ^[1] ; de Oliveira, Leandro F. ^[1] ; Piovezani, Amanda R. ^[2] ; dos Santos, Andre L. W. ^[1] ; de Souza, Diego T. ^[1] ; Demarco, Diego ^[3] ; Buckeridge, Marcos S. ^[2] ; Floh, Eny I. S. ^[1]

Afiliação do(s) autor(es)

^[1] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Sao Paulo, SP - Brazil

^[2] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Physiol Ecol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Anat, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT CELL TISSUE AND ORGAN CULTURE; v. 148, n. 1 SEP 2021.
• Citações Web of Science: 0

Resumo

The acquisition of embryogenic competence involves aspects at a cellular level related to cell communication and signaling. For Araucariaangustifolia, transcriptome and proteome databases have indicated differential gene expression and protein abundance related to cell-to-cell communication during embryogenesis. The differences are mainly related to vesicular transport and regulation of plasmodesmata region-related proteins. This study aims to understand the behavior of cell-to-cell communication in cell cultures with contrasting embryogenic potential. We used two embryogenic cell lines of A. angustifolia (Responsive and Blocked) and carried out gene expression, coexpression network, immunocytochemical, and ultrastructural analyses. Eight proteins related to vesicular transport and cell-to-cell communication processes were identified with a significantly different abundance in both cell lines. One callose hydrolase (AaGHL17) was differentially expressed between the two cell lines. Accordingly, we used immunocytochemistry and found a higher intensity of callose in the Responsive, as opposed to the Blocked embryogenic cells. Transmission Electron Microscopy images highlighted a more intense vesicular activity in the Responsive cell line compared to the Blocked one. Different status of cell-to-cell intercommunication and vesicular transport is associated with somatic embryogenic competence. Key message Vesicular transport and plasmodesmata regulation features are associated with Araucariaangustifolia somatic embryogenic responsiveness. (AU)

• Processo FAPESP: 16/17541-2 - Do estresse ao embrião: mecanismos moleculares e fisiológicos associados à embriogênese somática
• Beneficiário: Eny Iochevet Segal Floh
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/26888-0 - Marcadores bioquímicos associados ao metabolismo de carboidratos durante a embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Ktze.
• Beneficiário: Bruno Viana Navarro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto