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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cell-to-cell trafficking patterns in cell lines of Araucariaangustifolia (Brazilian pine) with contrasting embryogenic potential

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Author(s):
Navarro, V, Bruno ; Elbl, Paula [1] ; de Oliveira, Leandro F. [1] ; Piovezani, Amanda R. [2] ; dos Santos, Andre L. W. [1] ; de Souza, Diego T. [1] ; Demarco, Diego [3] ; Buckeridge, Marcos S. [2] ; Floh, Eny I. S. [1]
Total Authors: 9
Affiliation:
[1] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Sao Paulo, SP - Brazil
[2] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Physiol Ecol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Anat, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLANT CELL TISSUE AND ORGAN CULTURE; v. 148, n. 1 SEP 2021.
Web of Science Citations: 0
Abstract

The acquisition of embryogenic competence involves aspects at a cellular level related to cell communication and signaling. For Araucariaangustifolia, transcriptome and proteome databases have indicated differential gene expression and protein abundance related to cell-to-cell communication during embryogenesis. The differences are mainly related to vesicular transport and regulation of plasmodesmata region-related proteins. This study aims to understand the behavior of cell-to-cell communication in cell cultures with contrasting embryogenic potential. We used two embryogenic cell lines of A. angustifolia (Responsive and Blocked) and carried out gene expression, coexpression network, immunocytochemical, and ultrastructural analyses. Eight proteins related to vesicular transport and cell-to-cell communication processes were identified with a significantly different abundance in both cell lines. One callose hydrolase (AaGHL17) was differentially expressed between the two cell lines. Accordingly, we used immunocytochemistry and found a higher intensity of callose in the Responsive, as opposed to the Blocked embryogenic cells. Transmission Electron Microscopy images highlighted a more intense vesicular activity in the Responsive cell line compared to the Blocked one. Different status of cell-to-cell intercommunication and vesicular transport is associated with somatic embryogenic competence. Key message Vesicular transport and plasmodesmata regulation features are associated with Araucariaangustifolia somatic embryogenic responsiveness. (AU)

FAPESP's process: 16/17541-2 - From stress to embryos: molecular and physiological mechanisms associated to somatic embryogenesis
Grantee:Eny Iochevet Segal Floh
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
Grantee:Marcos Silveira Buckeridge
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/26888-0 - Biochemical markers associated with carbohydrate metabolism during Araucaria angustifolia zygotic and somatic embryogenesis
Grantee:Bruno Viana Navarro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)