Taurine upregulates insulin signaling and mitochondrial metabolism in vitro but not in adipocytes of obese women

Autor(es):Mostrar menos -

De Carvalho, Flavia G. ^[1] ; Munoz, Vitor R. ^[2] ; Brandao, Camila F. C. ^{[3, 4]} ; Simabuco, Fernando M. ^[5] ; Pavan, Isadora C. B. ^{[5, 6]} ; Nakandakari, Susana C. B. R. ^[7] ; Pauli, Jose Rodrigo ^[2] ; De Moura, Leandro P. ^[2] ; Ropelle, Eduardo R. ^[2] ; Marchini, Julio Sergio ^[3] ; da Silva, Adelino S. R. ^[1] ; de Freitas, Ellen C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Univ Estadual Campinas, Lab Mol Biol Exercise, Limeira - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Internal Med Dept, Ribeirao Preto - Brazil

^[4] Univ Estado Minas Gerais, Divinopolis Unit, Divinopolis - Brazil

^[5] Univ Estadual Campinas, Sch Appl Sci, Multidisciplinary Lab Food & Hlth, Limeira - Brazil

^[6] Univ Estadual Campinas, Sch Pharmaceut Sci, Lab Signaling Mech, Campinas - Brazil

^[7] Univ Estadual Campinas, Lab Nutr Genom, Limeira - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION; v. 93, JAN 2022.
• Citações Web of Science: 1

Resumo

Objectives: Based on taurine's beneficial roles in metabolic diseases in rodents and obese individuals, we investigated the effects of taurine supplementation on adipose tissue using transcriptome analysis, 3T3-L1 adipocytes, and subcutaneous white adipose tissue (scWAT) of obese women. Methods: First, we applied bioinformatics analysis to evaluate the effect of the taurine synthesis pathway on the adipose tissue of several BXD mice strains. After that, using 3T3-L1 adipocytes, we investigated the effects of different taurine doses in proteins related to insulin signaling, lipid oxidation, and mitochondrial function. Finally, we evaluated the effects of taurine supplementation (3 grams, 8 wk) on the same proteins in the scWAT of obese women. Results: The transcriptome analysis showed that the taurine biosynthesis pathway was positively associated with insulin signaling and mitochondrial metabolism in the scWAT of BXD mice. The experiments using 3T3-L1 cells highlighted that the taurine dosage has an essential function in taurine synthesis, insulin, and mitochondrial markers. In contrast, the 8-wk taurine administration did not change the basal insulin, proteins of the taurine synthesis or insulin pathways, lipid oxidation, or mitochondrial metabolism in the scWAT of obese women. Conclusions: For the first time, to our knowledge, we showed that supplementation with 3 g of taurine for 8 wk promoted no effect in the insulin signaling pathway in the scWAT of obese women. These findings bring new perspectives to investigate different taurine doses and the intervention period for human studies owing to the potential antiobesity activity of taurine. (c) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 17/08036-5 - Efeitos da suplementação de taurina associada ou não a prática de exercício concorrente sobre a função mitocondrial, gasto energético e perfil inflamatório de mulheres obesas
• Beneficiário: Flávia Giolo de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/10080-2 - Metabolismo energético mitocondrial, inflamação e estresse oxidativo em mulheres obesas submetidas a suplementação de taurina associado ao treinamento físico
• Beneficiário: Ellen Cristini de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Regular