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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Taurine upregulates insulin signaling and mitochondrial metabolism in vitro but not in adipocytes of obese women

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Author(s):
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De Carvalho, Flavia G. [1] ; Munoz, Vitor R. [2] ; Brandao, Camila F. C. [3, 4] ; Simabuco, Fernando M. [5] ; Pavan, Isadora C. B. [5, 6] ; Nakandakari, Susana C. B. R. [7] ; Pauli, Jose Rodrigo [2] ; De Moura, Leandro P. [2] ; Ropelle, Eduardo R. [2] ; Marchini, Julio Sergio [3] ; da Silva, Adelino S. R. [1] ; de Freitas, Ellen C. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil
[2] Univ Estadual Campinas, Lab Mol Biol Exercise, Limeira - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Internal Med Dept, Ribeirao Preto - Brazil
[4] Univ Estado Minas Gerais, Divinopolis Unit, Divinopolis - Brazil
[5] Univ Estadual Campinas, Sch Appl Sci, Multidisciplinary Lab Food & Hlth, Limeira - Brazil
[6] Univ Estadual Campinas, Sch Pharmaceut Sci, Lab Signaling Mech, Campinas - Brazil
[7] Univ Estadual Campinas, Lab Nutr Genom, Limeira - Brazil
Total Affiliations: 7
Document type: Journal article
Source: NUTRITION; v. 93, JAN 2022.
Web of Science Citations: 1
Abstract

Objectives: Based on taurine's beneficial roles in metabolic diseases in rodents and obese individuals, we investigated the effects of taurine supplementation on adipose tissue using transcriptome analysis, 3T3-L1 adipocytes, and subcutaneous white adipose tissue (scWAT) of obese women. Methods: First, we applied bioinformatics analysis to evaluate the effect of the taurine synthesis pathway on the adipose tissue of several BXD mice strains. After that, using 3T3-L1 adipocytes, we investigated the effects of different taurine doses in proteins related to insulin signaling, lipid oxidation, and mitochondrial function. Finally, we evaluated the effects of taurine supplementation (3 grams, 8 wk) on the same proteins in the scWAT of obese women. Results: The transcriptome analysis showed that the taurine biosynthesis pathway was positively associated with insulin signaling and mitochondrial metabolism in the scWAT of BXD mice. The experiments using 3T3-L1 cells highlighted that the taurine dosage has an essential function in taurine synthesis, insulin, and mitochondrial markers. In contrast, the 8-wk taurine administration did not change the basal insulin, proteins of the taurine synthesis or insulin pathways, lipid oxidation, or mitochondrial metabolism in the scWAT of obese women. Conclusions: For the first time, to our knowledge, we showed that supplementation with 3 g of taurine for 8 wk promoted no effect in the insulin signaling pathway in the scWAT of obese women. These findings bring new perspectives to investigate different taurine doses and the intervention period for human studies owing to the potential antiobesity activity of taurine. (c) 2021 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 17/08036-5 - Effects of taurine supplementation associated or not to exercise on mitochondrial function, energy expenditure and inflammatory profile in obese women
Grantee:Flávia Giolo de Carvalho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/10080-2 - Mitochondrial energy metabolism, inflammation and oxidative stress in obese women submitted to taurine supplementation and physical training
Grantee:Ellen Cristini de Freitas
Support Opportunities: Regular Research Grants