Bacteriophages M13 and T4 Increase the Expression of Anchorage-Dependent Survival Pathway Genes and Down Regulate Androgen Receptor Expression in LNCaP Prostate Cell Line

Autor(es)

Sanmukh, Swapnil Ganesh ^[1] ; dos Santos, Nilton Jose ^{[2, 1]} ; Barquilha, Caroline Nascimento ^{[2, 1]} ; Cucielo, Maira Smaniotto ^[1] ; de Carvalho, Marcio ^[3] ; dos Reis, Patricia Pintor ^[3] ; Delella, Flavia Karina ^[1] ; Carvalho, Hernandes F. ^[2] ; Felisbino, Sergio Luis ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, BR-18618689 Botucatu, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 13, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

Wild-type or engineered bacteriophages have been reported as therapeutic agents in the treatment of several types of diseases, including cancer. They might be used either as naked phages or as carriers of antitumor molecules. Here, we evaluate the role of bacteriophages M13 and T4 in modulating the expression of genes related to cell adhesion, growth, and survival in the androgen-responsive LNCaP prostatic adenocarcinoma-derived epithelial cell line. LNCaP cells were exposed to either bacteriophage M13 or T4 at a concentration of 1 x 10(5) pfu/mL, 1 x 10(6) pfu/mL, and 1 x 10(7) pfu/mL for 24, 48, and 72 h. After exposure, cells were processed for general morphology, cell viability assay, and gene expression analyses. Neither M13 nor T4 exposure altered cellular morphology, but both decreased the MTT reduction capacity of LNCaP cells at different times of treatment. In addition, genes AKT, ITGA5, ITGB1, ITGB3, ITGB5, MAPK3, and PI3K were significantly up-regulated, whilst the genes AR, HSPB1, ITGAV, and PGC1A were down-regulated. Our results show that bacteriophage M13 and T4 interact with LNCaP cells and effectively promote gene expression changes related to anchorage-dependent survival and androgen signaling. In conclusion, phage therapy may increase the response of PCa treatment with PI3K/AKT pathway inhibitors. (AU)

• Processo FAPESP: 19/19644-1 - Câncer de próstata: participação das vias da adiponectina e do colágeno tipo X
• Beneficiário: Sérgio Luis Felisbino
• Modalidade de apoio: Auxílio à Pesquisa - Regular