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Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance

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Autor(es):
Carvalho, Isis Gabriela Barbosa [1] ; Merfa, Marcus Vinicius [2] ; Teixeira-Silva, Natalia Sousa [1] ; Martins, Paula Maria Moreira [1] ; Takita, Marco Aurelio [1] ; de Souza, Alessandra Alves [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Ctr Citricultura Sylvio Moreira, Inst Agronom, Cordeiropolis - Brazil
[2] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 12, SEP 20 2021.
Citações Web of Science: 0
Resumo

Copper-based compounds are widely used in agriculture as a chemical strategy to limit the spread of multiple plant diseases; however, the continuous use of this heavy metal has caused environmental damage as well as the development of copper-resistant strains. Thus, it is important to understand how the bacterial phytopathogens evolve to manage with this metal in the field. The MqsRA Toxin-Antitoxin system has been recently described for its function in biofilm formation and copper tolerance in Xylella fastidiosa, a plant-pathogen bacterium responsible for economic damage in several crops worldwide. Here we identified differentially regulated genes by X. fastidiosa MqsRA by assessing changes in global gene expression with and without copper. Results show that mqsR overexpression led to changes in the pattern of cell aggregation, culminating in a global phenotypic heterogeneity, indicative of persister cell formation. This phenotype was also observed in wild-type cells but only in the presence of copper. This suggests that MqsR regulates genes that alter cell behavior in order to prime them to respond to copper stress, which is supported by RNA-Seq analysis. To increase cellular tolerance, proteolysis and efflux pumps and regulator related to multidrug resistance are induced in the presence of copper, in an MqsR-independent response. In this study we show a network of genes modulated by MqsR that is associated with induction of persistence in X. fastidiosa. Persistence in plant-pathogenic bacteria is an important genetic tolerance mechanism still neglected for management of phytopathogens in agriculture, for which this work expands the current knowledge and opens new perspectives for studies aiming for a more efficient control in the field.</p> (AU)

Processo FAPESP: 19/01447-5 - Obtenção de porta enxerto transgênico: possibilidade de aumento de níveis de resistência da copa a fitopatógenos através da translocação de moléculas difusíveis
Beneficiário:Natália de Sousa Teixeira e Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
Beneficiário:Marcos Antonio Machado
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/15741-4 - Caracterização dos genes alvo da toxina MqsR em Xylella fastidiosa: função regulatória e formação de células persistentes
Beneficiário:Isis Gabriela Barbosa Carvalho
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
Beneficiário:Alessandra Alves de Souza
Linha de fomento: Auxílio à Pesquisa - Temático