MiR-125a-3p and MiR-320b Differentially Expressed in Patients with Chronic Myeloid Leukemia Treated with Allogeneic Hematopoietic Stem Cell Transplantation and Imatinib Mesylate

Autor(es)

Martins, Juliana R. B. ^[1] ; Moraes, Leonardo N. ^[2] ; Cury, Sarah S. ^[3] ; Capannacci, Juliana ^[1] ; Carvalho, Robson Francisco ^[3] ; Nogueira, Celia Regina ^[1] ; Hokama, Newton Key ^[1] ; Hokama, Paula O. M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618687 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ FCA UNESP, Sch Agr, Dept Bioproc & Biotechnol, BR-18610034 Botucatu, SP - Brazil

^[3] Sao Paulo State Univ IBB UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 19 OCT 2021.
• Citações Web of Science: 0

Resumo

Chronic myeloid leukemia (CML), a hematopoietic neoplasm arising from the fusion of BCR (breakpoint cluster region) gene on chromosome 22 to the ABL (Abelson leukemia virus) gene on chromosome 9 (BCR-ABL1 oncogene), originates from a small population of leukemic stem cells with extensive capacity for self-renewal and an inflammatory microenvironment. Currently, CML treatment is based on tyrosine kinase inhibitors (TKIs). However, allogeneic hematopoietic stem cell transplantation (HSCT-allo) is currently the only effective treatment of CML. The difficulty of finding a compatible donor and high rates of morbidity and mortality limit transplantation treatment. Despite the safety and efficacy of TKIs, patients can develop resistance. Thus, microRNAs (miRNAs) play a prominent role as biomarkers and post-transcriptional regulators of gene expression. The aim of this study was to analyze the miRNA profile in CML patients who achieved cytogenetic remission after treatment with both HSCT-allo and TKI. Expression analyses of the 758 miRNAs were performed using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Bioinformatics tools were used for data analysis. We detected miRNA profiles using their possible target genes and target pathways. MiR-125a-3p stood out among the downregulated miRNAs, showing an interaction network with 52 target genes. MiR-320b was the only upregulated miRNA, with an interaction network of 26 genes. The results are expected to aid future studies of miRNAs, residual leukemic cells, and prognosis in CML.</p> (AU)

• Processo FAPESP: 11/50629-7 - MicroRNAs circulantes como novos marcadores prognósticos em leucemia mielóide crônica
• Beneficiário: Paula de Oliveira Montandon Hokama
• Modalidade de apoio: Auxílio à Pesquisa - Regular