alidation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carrier

Autor(es)

Sousa Araujo, Victor Hugo ^[1] ; Fernandes, Ligia de Souza ^[2] ; Dos Reis, Leidiana Rocha ^[1] ; Carvalho, Gabriela Correa ^[1] ; Scarpa, Maria Virginia ^[2] ; Chorilli, Marlus ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 44, n. 23 OCT 2021.
• Citações Web of Science: 0

Resumo

Vulvovaginal candidiasis is a public health problem with a high incidence among female patients. Currently, there is an increase in the identification of Candida spp. resistant to current therapy, making it necessary to search for new therapeutic alternatives. The synergistic potential of curcumin with fluconazole is described in the literature. However, due to its high lipophilicity, it is necessary to use drug-delivery systems to adequately explore its potential, among which is the nanostructured lipid carrier. However, to date, there is no validated method of high-performance liquid chromatography for simultaneous determination of fluconazole and curcumin in the literature. Thus, the present work developed a high-performance liquid chromatography method for simultaneous determination of fluconazole and curcumin co-encapsulated in nanostructured lipid carrier which was validated according to the International Conference on Harmonization (Technical Requirements for Registration of Pharmaceuticals for Human Use) - Q2 (R1) and the Food and Drug Administration - Guidance for Bioanalytical Method. The method was applied to determine the encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. The developed method proved to be selective, precise, accurate, and robust for the simultaneous determination of both drugs, enabling the quantification of encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. (AU)

• Processo FAPESP: 19/10261-2 - Avaliação do potencial de carreadores lipídicos nanoestruturados para co-encapsulação de curcumina e fluconazol dispersos em hidrogéis termorreponsivos no tratamento de candidíase vulvovaginal
• Beneficiário: Victor Hugo Sousa Araujo
• Modalidade de apoio: Bolsas no Brasil - Doutorado