Neutrophil-suppressive activity over T-cell proliferation and fungal clearance in a murine model of Fonsecaea pedrosoi infection

Autor(es)

Dantas Breda, Leandro Carvalho ^{[1, 2]} ; de Souza Breda, Cristiane Naffah ^[1] ; Kaihami, Gilberto Hideo ^[3] ; Fogaca de Almeida, Jose Roberto ^[2] ; Jannuzzi, Grasielle Pereira ^[2] ; Ferreira, Lucas Goncalves ^[2] ; de Almeida, Sandro Rogerio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 OCT 12 2021.
• Citações Web of Science: 0

Resumo

Neutrophils are essential to control several fungal infections. These cells are commonly known for their pro-inflammatory activities. However, some studies have demonstrated the anti-inflammatory properties of neutrophils during certain infectious diseases, culminating in the inhibition of T cell proliferation. Chromoblastomycosis (CBM) is a deep and progressive mycosis that affects thousands of people worldwide. Although neutrophil infiltrates are observed in the lesion histopathology, the fungus can overtake the immune system response and destroy the host-infected tissue. The present study demonstrated that neutropenic animals had an increase in the IL-6 production in the spleen and liver, followed by a lower fungal burden in these organs up to 14 days of infection. Neutropenic animals also showed a lower F. pedrosoi-specific antibody production 14-days post infection and higher T-cell proliferation in the in vitro experiments after stimulation with F. pedrosoi-purified proteins. Taken together, our results suggest that the presence of regulatory neutrophils in the mouse model of F. pedrosoi infection could act favoring the spread of the fungus and the chronicity of the infection. These findings shed light on the CBM treatment, which might target neutrophil polarization as a new therapy approach to treat CBM lesions. (AU)

• Processo FAPESP: 19/14347-9 - Estudo das funções efetoras e do metabolismo de macrófagos e neutrófilos na eliminação do fungo Fonsecaea pedrosoi
• Beneficiário: Leandro Carvalho Dantas Breda
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/02708-7 - Estudo do metabolismo e do papel do itaconato na regulação das funções efetoras de macrófagos e neutrófilos infectados por Fonsecaea pedrosoi
• Beneficiário: Sandro Rogerio de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/11146-9 - O papel dos neutrófilos e dos receptores TLR 2 e TLR 4 na cromoblastomicose experimental em infecção por Fonsecaea pedrosoi
• Beneficiário: Leandro Carvalho Dantas Breda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/18598-7 - Papel de receptores de reconhecimento padrão (PRRs) na ativação de macrófagos nas infecções fúngicas cutâneas causadas por Fonsecaea pedrosoi, Sporothrix schenckii e Trichophyton rubrum
• Beneficiário: Sandro Rogerio de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Temático