Synbiotic supplementation attenuates the promoting effect of indole-3-carbinol on colon tumorigenesis

Autor(es)

de Moura, N. A. ^[1] ; Caetano, B. F. R. ^[2] ; Bidinotto, L. T. ^{[3, 4, 2]} ; Rodrigues, M. A. M. ^[2] ; Barbisan, L. F. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Prof Dr Antonio Celso Wagner Zanin 250, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Med, Dept Pathol, Botucatu, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil

^[4] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BENEFICIAL MICROBES; v. 12, n. 5, p. 493-501, 2021.
• Citações Web of Science: 0

Resumo

Indole-3 carbinol (I3C) has shown dual effects on the promotion and progression stages of colon carcinogenesis while synbiotics (Syn) have exerted anti-carcinogenic activities in most rodent studies. This study aimed to investigate the effects of I3C given alone or together with a Syn intervention on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. All animals were given four subcutaneous DMH injections (4x40 mg/kg bodyweight, twice a week for two weeks) and then received either basal diet (G1), basal diet containing I3C (1g/kg chow) (G2) or basal diet containing I3C+Syn (I3C + inulin 50g/kg chow + Bifidobacterium lactis BB-12 (R)), 2.5x10(10) cfu/g of basal diet), (G3) for 21 weeks. Dietary I3C (G2) significantly increased tumour volume and cell proliferation when compared to the DMH control group (G1). Syn intervention (G3) significantly reduced tumour volume and cell proliferation when compared to I3C (G2). The colon tumours found were classified into well-differentiated tubular adenomas or adenocarcinomas. Dietary I3C or I3C+Syn did not significantly affect the incidence and the multiplicity of tumours in comparison with the DMH control group. Furthermore, Syn intervention (G3) increased Gstm1 and reduced Mapk9 gene expression in colonic tumours. The findings of the present study show that the dietary I3C shows a weak promoting activity, while the combination with Syn ameliorates I3C effects. (AU)

• Processo FAPESP: 13/08033-5 - Genotoxicidade da água fecal e desenvolvimento de focos de criptas aberrantes em ratos Wistar alimentados com heme, indol-3-carbinol e/ou simbiótico
• Beneficiário: Brunno Felipe Ramos Caetano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/12631-2 - Efeitos de simbióticos e indol 3-carbinol na expressão de genes relacionados à carcinogênese de cólon em ratos alimentados com dieta contendo heme
• Beneficiário: Luís Fernando Barbisan
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23699-4 - Efeitos da ingestão de simbiótico e indol 3- carbinol sobre o processo de carcinogênese química de cólon em ratos Wistar alimentados com dieta contendo heme.
• Beneficiário: Nelci Antunes de Moura
• Modalidade de apoio: Bolsas no Brasil - Doutorado