Synbiotic supplementation attenuates the promoting effect of indole-3-carbinol on colon tumorigenesis

Author(s)

de Moura, N. A. ^[1] ; Caetano, B. F. R. ^[2] ; Bidinotto, L. T. ^{[3, 4, 2]} ; Rodrigues, M. A. M. ^[2] ; Barbisan, L. F. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Prof Dr Antonio Celso Wagner Zanin 250, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Med, Dept Pathol, Botucatu, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil

^[4] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil

• Document type: Journal article
• Source: BENEFICIAL MICROBES; v. 12, n. 5, p. 493-501, 2021.
• Web of Science Citations: 0

Abstract

Indole-3 carbinol (I3C) has shown dual effects on the promotion and progression stages of colon carcinogenesis while synbiotics (Syn) have exerted anti-carcinogenic activities in most rodent studies. This study aimed to investigate the effects of I3C given alone or together with a Syn intervention on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. All animals were given four subcutaneous DMH injections (4x40 mg/kg bodyweight, twice a week for two weeks) and then received either basal diet (G1), basal diet containing I3C (1g/kg chow) (G2) or basal diet containing I3C+Syn (I3C + inulin 50g/kg chow + Bifidobacterium lactis BB-12 (R)), 2.5x10(10) cfu/g of basal diet), (G3) for 21 weeks. Dietary I3C (G2) significantly increased tumour volume and cell proliferation when compared to the DMH control group (G1). Syn intervention (G3) significantly reduced tumour volume and cell proliferation when compared to I3C (G2). The colon tumours found were classified into well-differentiated tubular adenomas or adenocarcinomas. Dietary I3C or I3C+Syn did not significantly affect the incidence and the multiplicity of tumours in comparison with the DMH control group. Furthermore, Syn intervention (G3) increased Gstm1 and reduced Mapk9 gene expression in colonic tumours. The findings of the present study show that the dietary I3C shows a weak promoting activity, while the combination with Syn ameliorates I3C effects. (AU)

• FAPESP's process: 13/08033-5 - Fecal water genotoxicity and development of aberrant crypt foci in male Wistar rats fed heme, indole-3-carbinol and/or symbiotic formulation
• Grantee: Brunno Felipe Ramos Caetano
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/12631-2 - Effects of symbiotic and indole 3-carbinol on the gene expression involved in colon carcinogenesis in rats fed a diet containing heme
• Grantee: Luís Fernando Barbisan
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23699-4 - Effects of symbiotic and indole-3-carbinol intake on colon carcinogenesis process in Wistar rats fed a diet containing heme.
• Grantee: Nelci Antunes de Moura
• Support Opportunities: Scholarships in Brazil - Doctorate