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Prostate fractal analysis in rats submitted to hormonal carcinogenesis and exposed to bisphenol A and phytoestrogens

Grant number: 19/14081-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): August 31, 2020
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Leonardo de Oliveira Mendes
Grantee:Thainá Cavalleri Sousa
Home Institution: Faculdade de Artes, Ciências, Letras e Educação de Presidente Prudente (FACLEPP). Universidade do Oeste Paulista (UNOESTE). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

Various chemical substances present in the environment are targets of studies due to their direct action on hormone-dependent tissues, such as Bisphenol A (BPA). This substance has one of the largest exposure spectra in the world, as well as being related to the development of tumors. On the other hand, some phytochemicals found in plants, such as Indol-3-Carbinol (I3C) and Genistein (GEN), have a chemoprotective effect on tumor cells through the activation of pro-apoptotic and antiproliferative signaling pathways. Thus, the present study aims to evaluate the morphological aspects of prostatic chemoprotection by I3C and GEN phytochemicals in male rats that were exposed during gestation and lactation to Bisphenol A and submitted to hormonal carcinogenesis. Female Sprague-Dawley pregnant females were divided into 5 experimental groups: C: Control; B: BPA (10 ¼g / kg); BG: BPA (10 ¼g / kg) + GEN (5 mg / kg); BIG: BPA (10 ¼g / kg) + I3C (20 mg / kg) + GEN (5 mg / kg); BI: BPA (10 ¼g / kg) + I3C (20 mg / kg). Treatments were performed on gestational day 17 gestation mothers until postnatal day (PND) 21. The animals received phytoestrogen-free basal ration and filtered water ad libitum. The selected males were euthanized in DPN 300. From the 90 days of age (DPN90) until the end of the experiment (DPN300), all animals received twice-weekly subcutaneous injections containing testosterone and estradiol at concentrations of 25 mg / day and 2.5 mg / day, respectively. After euthanasia in the DPN300, the ventral prostate (PV) of the animals were collected, dissected and are currently included in paraplast. Considering that histopathological studies focus mainly on qualitative analyzes, it is necessary to investigate quantitative and non-subjective prostatic tissue remodeling. For this, the fractal analysis will be performed on slides submitted to staining with Hematoxylin-Eosin and Picrossyrius-Hematoxylin. In addition, the relative volume of collagen in Picrossíruis-Hematoxilina-stained slides and the immunolocalization of type I and type III collagen in the prostatic stroma through immunohistochemical analysis will be quantified.