Searching for bioactive compounds from Solanaceae: lethal and sublethal toxicity to Spodoptera frugiperda and untargeted metabolomics approaches

Autor(es)

Lima, Andreisa Fabri ^{[1, 2]} ; Ribeiro, Leandro do Prado ^[3] ; Padoan Goncalves, Gabriel Luiz ^[4] ; Maimone, Naydja Moralles ^[4] ; Gissi, Danilo Soares ^[5] ; de Lira, Simone Possedente ^[4] ; Vendramim, Jose Djair ^[2]

Afiliação do(s) autor(es)

^[1] Lavras Fed Univ UFLA, Dept Entomol, Lavras, MG - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Entomol & Acarol, USP, ESALQ, Piracicaba, SP - Brazil

^[3] Agr Res & Rural Extens Co Santa Catarina EPAGRI C, Res Ctr Family Agr, Rua Ferdinando Ricieri Tusset S-N, BR-89801970 Chapeco, SC - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Exact Sci, USP, ESALQ, Piracicaba, SP - Brazil

^[5] Sao Paulo State Univ, Dept Bot, UNESP, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PEST SCIENCE; NOV 2021.
• Citações Web of Science: 0

Resumo

The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a major pest of maize crops and others row crops on the American continent, and this pest has spread to Africa and Asia. Such lepidopteran pest presents resistant populations to synthetic insecticides and to Bacillus thuringiensis (Bt) toxins and, consequently, new (bio) insecticides with distinct modes of action are required. In order to search for derivatives from Solanaceae as a potential source of bioactive compounds, a screening of 38 ethanolic extracts were prepared from 25 Solanaceae species. Using dietary exposure assessment, the ethanolic extracts from leaves of Acnistus arborescens (L.) Schltdl. and Datura stramonium L. were the most promising derivatives. A bioguided fractionation was performed with both plant species. The A. arborescens dichloromethane fraction was the most active, causing sublethal (growth inhibition) and lethal toxicity. Thus, this fraction was submitted to another fractionation procedure resulting in three subfractions (dichloromethane, ethyl acetate and methanol) that were tested against S. frugiperda caterpillars. All subfractions lead to significant sublethal effects, however, without significant mortality. The chemical diversity in the subfractions of A. arborescens was assessed through untargeted approaches, such as molecular networking and the in silico annotation tool NAP, by which two withanolides glycosides were annotated: (22R)-1-Oxo-3beta-(beta-D-glucopyranosyloxy)-14,20,22,27-tetrahydroxyerg osta-5,24-diene-26-oic acid delta-lactone and withanoside XI. Therefore, derivatives of Solanaceae present promising compounds that may be useful in the framework of S. frugiperda integrated pest management. In addition, metabolomics is an innovative alternative and powerful approach to facilitate the putative identification of compounds in studies of natural products. (AU)

• Processo FAPESP: 13/50228-8 - Componentes da biodiversidade, e seus caracteres metabólicos, de ilhas do Brasil: uma abordagem integrada
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 09/54094-0 - EMU: aquisição de um espectrômetro de massas de estágios múltiplos para estruturação do Laboratório / Facilities do Departamento de Química da FFCLRP-USP
• Beneficiário: Luiz Alberto Beraldo de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários