Differential Expression of MMP2 and TIMP2 in Peripheral Blood Mononuclear Cells After Roux-en-Y Gastric Bypass

Autor(es)

Nonino, Carla Barbosa ^[1] ; Noronha, Natalia Yumi ^[1] ; de Araujo Ferreira-Julio, Maysa ^[2] ; Moriguchi Watanabe, Ligia ^[1] ; Cassia, Karen Francislaine ^[2] ; Ferreira Nicoletti, Carolina ^[1] ; Rossi Welendorf, Caroline ^[1] ; Salgado Junior, Wilson ^[3] ; Rossi Silva Souza, Doroteia ^[2] ; Augusta de Souza Pinhel, Marcela ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Ribeirao Preto - Brazil

^[2] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Sao Jose Do Rio Preto - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN NUTRITION; v. 8, OCT 13 2021.
• Citações Web of Science: 0

Resumo

Matrix metalloproteinases (MMP) and their endogenous inhibitor, the tissue inhibitor of metalloproteinases (TIMP), are expressed in many different cell types and play an important role in physiologic and pathological degradation of extracellular matrix (ECM). Starting from these observations and considering the activation state of peripheral blood mononuclear cells (PBMCs) in obesity, we investigated the gene expression of metalloproteinases before and after Roux-en-Y gastric bypass (RYBG). The study was performed in the Ribeirao Preto Medical School University Hospital. Seventy-three women were divided into a study group (SG), composed of 53 individuals with severe obesity before and after 6 months of RYGB, and a control group (CG), composed of 20 normal-weight individuals. Anthropometric and body composition data were collected, and peripheral blood for ribonucleic acid (RNA) extraction. The biological samples were submitted to a quantitative real-time polymerase chain reaction to evaluate the expression of MMP2 and TIMP2 genes. Alterations in weight loss, body mass index (BMI), and fat mass (FM) were observed after 6 months of RYGB (p < 0.05). A reduction of gene expression of TIMP2 was observed after 6 months of RYGB, contributing positively to the weight loss (R-2 = 0.33 p = 0.04). The enrichment analyses highlighted the interaction between TIMP2 and MMP2 genes and the molecular pathways involving the ECM remodeling in the obesity condition. RYGB contributes significantly to weight loss, improved BMI, reduced FM, and reduced TIMP2 expression in PBMCs, which might contribute to the ECM remodeling in the obesity and could be useful as a circulating biomarker.</p> (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/12819-4 - Alterações genéticas, clínicas, bioquímicas e de variabilidade cardíaca na Obesidade grau III pré e pós-cirurgia bariátrica
• Beneficiário: Marcela Augusta de Souza Pinhel
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado