Histidine-based hydrogels via singlet-oxygen photooxidation

Autor(es)

Liberato, Michelle S. ^[1] ; Cavalcante, Nayara G. S. ^[1] ; Sindu, P. Abinaya ^[1] ; Rodrigues-Jesus, Monica J. ^[2] ; Zelenovskii, Pavel ^{[3, 4]} ; Carreira, Ana C. O. ^[5] ; Baptista, Mauricio S. ^[6] ; Sogayar, Mari C. ^{[1, 5]} ; Ferreira, Luis C. S. ^[2] ; Catalani, Luiz H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo - Brazil

^[3] Univ Aveiro, Dept Chem, P-3810193 Aveiro - Portugal

^[4] Univ Aveiro, CICECO Aveiro Inst Mat, P-3810193 Aveiro - Portugal

^[5] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL, Med Sch, BR-05508220 Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SOFT MATTER; v. 17, n. 48 NOV 2021.
• Citações Web of Science: 0

Resumo

The formation of hydrogels by photosensitized oxidation and crosslinking of histidine-derived polymers is demonstrated for the first time. The photooxidation of pendant His mediated by singlet oxygen was used to promote covalent coupling by its dimerization. As a proof-of-concept, two systems were studied: (i) chondroitin sulfate (CS) functionalized with His, and (ii) an elastin-like peptide (ELP) containing His produced by recombinant techniques. Both materials were crosslinked by irradiation at 425 nm in the presence of Zn-porphyrin derivatives yielding His-based hydrogels. The molecular structure and physicochemical properties of ELP-His and other 5 ELPs with photooxidizable amino acids were studied in silica by computer simulation. A correlation between the protein conformation and its elastic properties is discussed. CS-His hydrogels demonstrate larger storage moduli than ELPs with other amino acids. The obtained results show the potential use of photooxidation to create a new type of His-based hydrogels. (AU)

• Processo FAPESP: 18/13492-2 - Arcabouços sintéticos e naturais aplicados à medicina regenerativa
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/08975-7 - EMU concedido no processo 2018/15539-6: reômetro modular compacto e acessórios
• Beneficiário: Fabiana Perrechil Bonsanto
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 19/10789-7 - Arcabouço celular inédito a partir de proteína recombinante derivada de elastina rica em histidina para impressão 3D
• Beneficiário: Michelle da Silva Liberato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado