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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histidine-based hydrogels via singlet-oxygen photooxidation

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Author(s):
Liberato, Michelle S. [1] ; Cavalcante, Nayara G. S. [1] ; Sindu, P. Abinaya [1] ; Rodrigues-Jesus, Monica J. [2] ; Zelenovskii, Pavel [3, 4] ; Carreira, Ana C. O. [5] ; Baptista, Mauricio S. [6] ; Sogayar, Mari C. [1, 5] ; Ferreira, Luis C. S. [2] ; Catalani, Luiz H. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo - Brazil
[3] Univ Aveiro, Dept Chem, P-3810193 Aveiro - Portugal
[4] Univ Aveiro, CICECO Aveiro Inst Mat, P-3810193 Aveiro - Portugal
[5] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL, Med Sch, BR-05508220 Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: SOFT MATTER; v. 17, n. 48 NOV 2021.
Web of Science Citations: 0
Abstract

The formation of hydrogels by photosensitized oxidation and crosslinking of histidine-derived polymers is demonstrated for the first time. The photooxidation of pendant His mediated by singlet oxygen was used to promote covalent coupling by its dimerization. As a proof-of-concept, two systems were studied: (i) chondroitin sulfate (CS) functionalized with His, and (ii) an elastin-like peptide (ELP) containing His produced by recombinant techniques. Both materials were crosslinked by irradiation at 425 nm in the presence of Zn-porphyrin derivatives yielding His-based hydrogels. The molecular structure and physicochemical properties of ELP-His and other 5 ELPs with photooxidizable amino acids were studied in silica by computer simulation. A correlation between the protein conformation and its elastic properties is discussed. CS-His hydrogels demonstrate larger storage moduli than ELPs with other amino acids. The obtained results show the potential use of photooxidation to create a new type of His-based hydrogels. (AU)

FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
Grantee:Luiz Henrique Catalani
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/08975-7 - Multi-User Equipment approved in grant 2018/15539-6: modular compact rheometer and accessories
Grantee:Fabiana Perrechil Bonsanto
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 19/10789-7 - Novel cellular scaffold from a histidine rich recombinant elastin-derived protein for 3D printing
Grantee:Michelle da Silva Liberato
Support Opportunities: Scholarships in Brazil - Post-Doctoral