Synthetic and natural scaffolds applied to regenerative medicine

• Grant number: 18/13492-2
• Support Opportunities: Research Projects - Thematic Grants
• Start date: February 01, 2019
• End date: January 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Henrique Catalani
• Grantee: Luiz Henrique Catalani
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Denise Freitas Siqueira Petri ; Susana Inés Córdoba de Torresi
• Associated researchers: Alexander Henning Ulrich ; Alexandre Bruni Cardoso ; Daniel Reinaldo Cornejo ; Flavia Vischi Winck ; José Eduardo Krieger ; Mari Cleide Sogayar ; Mauricio da Silva Baptista ; Roberto Manuel Torresi ; Silvya Stuchi Maria-Engler
• Associated research grant(s): 22/13203-6 - Multi-user equipment approved in the grant 2018/13492-2: rheometer and accessories, AP.EMU; 19/22671-0 - Peptides-polysaccharides conjugates as antimicrobial materials for 3D printing, AP.R SPRINT; 19/15781-4 - Study of adhesion and proliferation of stem cells in capacitive electrical stimuli over scaffolds in 2D and 3D made by electrospinning and Solution Blow Spinning, AP.R SPRINT; 19/08178-0 - Multi-User Equipment approved in grant 2018/13492-2: atomic force microscope, AP.EMU
• Associated scholarship(s): 22/16427-2 - Photo-crosslinkable N-carboxyanhydride derived polypeptides with pseudoplastic characteristics for extrusion-based 3D printing, BP.PD; 22/13146-2 - Biomaterials based on conductive and biodegradable Polymers and bionanoplasmonics, BP.PD; 22/11678-7 - Development of gel permeation chromatography (GPC) techniques, BP.TT; + associated scholarships 22/01784-4 - Atomic force microscopy for the characterization of scaffolds: topography, adhesion forces, BP.TT; 22/01164-6 - Biomaterials based on biodegradable conducting polymers and bioplasmonics, BP.PD; 21/14039-2 - Development of gel permeation chromatography (GPC) techniques, BP.TT; 21/12024-8 - Atomic force microscopy for the characterization of scaffolds: topography, adhesion forces, elasticity modulus, electric potential, BP.TT; 21/00490-4 - Study of functional and photoreticulable hydrogels derived from biopolymers for application in 3D printing, BP.DD; 20/15986-2 - Synthesis and characterization of photocrosslinkable and functionalizable polypeptides for 3D print, BP.DD; 20/15005-1 - A solvent-free route for the synthesis of the composite AuNPs@PEDOT-co-PDLLA e its biomedical applications, BP.IC; 20/03248-7 - Study on the release of caffeine from magnetic xanthan scaffolds under magnetic stimulus, BP.IC; 20/05632-9 - Nanotopography and nanomechanical properties of nanostructured chitosan scaffolds and of cells adhered on them, BP.DR; 20/01907-3 - Determination of mechanical properties of polysaccharide-based magnetic scaffolds, BP.PD; 19/20405-1 - Techniques development on gel permeation chromatography (GPC), BP.TT; 19/19980-1 - Development of catalytic scaffolds, BP.IC; 19/10789-7 - Novel cellular scaffold from a histidine rich recombinant elastin-derived protein for 3D printing, BP.PD; 19/12466-0 - Biodegradavel and biocompativel materials based on conducting polymers, BP.IC; 19/12962-8 - Synthesis of polyesters based on panthenol via chemical catalysis, BP.IC; 19/05732-6 - Synthetic and natural scaffolds applied to regenerative medicine, BP.TT; 19/06677-9 - Study of lysozyme adsorption and desorption in layer-by-layer films by means of FRET, BP.IC; 19/02669-1 - Quartz crystal microbalance with dissipation for characterization of adsorption/adhesion processes of proteins and cells on biomaterials, BP.TT - associated scholarships

Abstract

This project has as its general goal the development of scaffolds for cellular growth from novel polymer systems, aiming at applications in biomedical engineering, especially in the area of regenerative medicine. We propose the combination of the efforts of specialists from various areas toward a multi- and interdisciplinary approach, in the solution of problems involving the control of several fundamental properties of the materials here used in the generation of artificial tissues. Hence, this project proposes the generation of scaffolds that exhibits chemical, physical and biological stimuli, aimed at the control of cellular responses, such as adhesion, proliferation, and differentiation. Among the chemical stimuli, we propose the functionalization of the surfaces with layer-by-layer systems, to include protein signaling molecules, as well as the use of decellularized and fractions of a digested extracellular matrix in the production of hybrid scaffolds. As physical stimuli, we propose the study of scaffolds produced with conducting polymers and magnetic nanoparticles, to examine how they can control the various biological events, and what mechanisms are involved. Also, we intend to add to the associated laboratories with last generation processing techniques, such as electrospinning and 3D printing, allowing picturing the role of morphology in biological events. To make the best use of these processing techniques, we propose the study of novel materials, with thermoset and photo-curing properties. In this way, it is necessary to provide adequate microscopic techniques capable of producing mechanical and conductive analyzes of the surfaces thus generated. The essence of the project is the integration of distinct but complementary approaches to understanding the complex relationship between material, architecture and cellular response. (AU)

Scientific publications

(39)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TUNDISI, L. L.; MOSTACO, G. B.; CARRICONDO, P. C.; PETRI, D. F. S.. Hydroxypropyl methylcellulose: Physicochemical properties and ocular drug delivery formulations. European Journal of Pharmaceutical Sciences, v. 159, APR 1 2021. (18/13492-2)

DELECHIAVE, GIOVANNE; NAVES, ALLINY F.; KOLANTHAI, ELAYARAJA; DA SILVA, RUBENS A.; VLASMAN, RAPHAEL C.; PETRI, DENISE F. S.; TORRESI, ROBERTO M.; CATALANI, LUIZ H.. Tuning protein delivery from different architectures of layer-by-layer assemblies on polymer films. MATERIALS ADVANCES, v. 1, n. 6, p. 2043-2056, SEP 1 2020. (18/14532-8, 11/21442-6, 17/06394-1, 15/19694-8, 18/13492-2, 15/10009-0)

MENESES, IZABO PEREIRA; NOVAES, STEPHANIE DIAS; DEZOTTI, RAFAEL SOBRAL; OLIVEIRA, PEDRO VITORIANO; SIQUEIRA PETRI, DENISE FREITAS. CTAB-modified carboxymethyl cellulose/bagasse cryogels for the efficient removal of bisphenol A, methylene blue and Cr(VI) ions: Batch and column adsorption studies. JOURNAL OF HAZARDOUS MATERIALS, v. 421, JAN 5 2022. (18/13492-2)

CHIAREGATO, CAMILA GRUBER; BERNARDINELLI, OIGRES DANIEL; SHAVANDI, AMIN; SABADINI, EDVALDO; PETRI, DENISE FREITAS SIQUEIRA. The effect of the molecular structure of hydroxypropyl methylcellulose on the states of water, wettability, and swelling properties of cryogels prepared with and without CaO2. Carbohydrate Polymers, v. 316, p. 10-pg., 2023-05-20. (18/13492-2, 22/06284-0)

ALAVARSE, ALEX CARVALHO; FRACHINI, EMILLI CAROLINE GARCIA; DA SILVA, RAFAEL LEONARDO CRUZ GOMES; LIMA, VITORIA HASHIMOTO; SHAVANDI, AMIN; PETRI, DENISE FREITAS SIQUEIRA. Crosslinkers for polysaccharides and proteins: Synthesis conditions, mechanisms, and crosslinking efficiency, a review. International Journal of Biological Macromolecules, v. 202, p. 39-pg., 2022-01-25. (20/03248-7, 20/01907-3, 19/22671-0, 20/05632-9, 18/13492-2)

TOLEDO, PAULO V. O.; BERNARDINELLI, OIGRES D.; SABADINI, EDVALDO; PETRI, DENISE F. S.. The states of water in tryptophan grafted hydroxypropyl methylcellulose hydrogels and their effect on the adsorption of methylene blue and rhodamine B. Carbohydrate Polymers, v. 248, p. 10-pg., 2020-11-15. (18/13492-2)

FURTADO, LAISE M.; YEE, MARCIO; FERNANDES, RODRIGO; VALERA, TICIANE S.; ITRI, ROSANGELA; PETRI, DENISE F. S.. Rheological and mechanical properties of hydroxypropyl methylcellulose-based hydrogels and cryogels controlled by AOT and SDS micelles. Journal of Colloid and Interface Science, v. 648, p. 12-pg., 2023-06-12. (18/13492-2)

MACHADO, RAPHAEL C. L.; FONSECA, KARINA T.; TEIXEIRA, VERONICA C.; CATALANI, LUIZ HENRIQUE; RODRIGUES, LUCAS C. V.. Development of a red persistent luminescent composite: Electrospun nanofiber polymer coating prevents emission quenching by water. MATERIALS TODAY COMMUNICATIONS, v. 35, p. 9-pg., 2023-04-21. (18/13492-2, 21/13791-2, 21/05603-1)

DA SILVA, RAFAEL L. C. G.; BERNARDINELLI, OIGRES D.; FRACHINI, EMILLI C. G.; ULRICH, HENNING; SABADINI, EDVALDO; PETRI, DENISE F. S.. Vanillin crosslinked chitosan films: The states of water and the effect of carriers on curcumin uptake. Carbohydrate Polymers, v. 292, p. 10-pg., 2022-06-14. (20/05632-9, 18/13492-2)

DA SILVA, ARUA CLAYTON; PASCHOAL, VITOR HUGO; RIBEIRO, MAURO CARLOS COSTA; DE TORRESI, SUSANA INES CORDOBA. Electrical/Spectroscopic Stability of Conducting and Biodegradable Graft-Copolymer. MACROMOLECULAR CHEMISTRY AND PHYSICS, v. 223, n. 19, p. 12-pg., 2022-08-15. (19/00207-0, 14/09353-6, 17/00705-5, 18/13492-2, 16/21070-5, 15/26308-7)

DA SILVA, RAFAEL T. P.; DE BARROS, HELOISE. R. R.; FERNANDES, RAFAELLA. F. F.; TEMPERINI, MARCIA L. A.; DE TORRESI, SUSANA. I. CORDOBA I.. Cytochrome C with Peroxidase-like Activity Supported on Plasmonic AuNPs: Improved Stability and Enhanced Nanobioplasmonic Catalytic Conversion. CHEMCATCHEM, v. N/A, p. 9-pg., 2023-02-01. (18/25422-9, 19/09668-0, 18/13492-2, 16/21070-5, 17/20892-4, 15/26308-7)

ALAVARSE, ALEX CARVALHO; SILVA, JEAN BEZERRA; ULRICH, HENNING; SIQUEIRA PETRI, DENISE FREITAS. Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains. SOFT MATTER, v. 19, n. 14, p. 11-pg., 2023-03-15. (18/13492-2, 18/07366-4, 20/01907-3, 19/08178-0)

BRAGA, VICTOR H. A.; FUENTES, DAIRON P.; OLIVEIRA, PEDRO, V; PETRI, DENISE F. S.. Biopolymer cryogels for enhanced adsorption of valuable metals from multi-metal waste after flocculation and precipitation. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 12, n. 5, p. 12-pg., 2024-10-01. (09/08584-6, 18/13492-2)

FURTADO, LAISE MOURA; FUENTES, DAIRON PEREZ; ANDO, ROMULO AUGUSTO; OLIVEIRA, PEDRO VITORIANO; SIQUEIRA PETRI, DENISE FREITAS. Carboxymethyl cellulose/sugarcane bagasse/polydopamine adsorbents for efficient removal of Pb²⁺ ions from synthetic and undergraduate laboratory wastes. JOURNAL OF CLEANER PRODUCTION, v. 380, p. 13-pg., 2022-12-20. (18/13492-2)

ALAVARSE, ALEX C.; DA SILVA, RAFAEL L. C. G.; GHAFFARI BOHLOULI, PEJMAN; CORNEJO, DANIEL; ULRICH, HENNING; SHAVANDI, AMIN; PETRI, DENISE F. S.. Magnetic Force Microscopy and Nanoindentation on 3D Printed Magnetic Scaffolds for Neuronal Cell Growth. ACS APPLIED POLYMER MATERIALS, v. 6, n. 2, p. 12-pg., 2023-12-26. (19/22671-0, 20/01907-3, 22/08442-1, 18/07366-4, 18/13492-2)

BARROS, HELOISE RIBEIRO; TANAKA, LIVIA YUKARI; PACHECO DA SILVA, RAFAEL TRIVELLA; SANTIAGO-ARCOS, JAVIER; CORDOBA TORRESI, I, SUSANA; LOPEZ-GALLEGO, FERNANDO. Assembly of Nano-Biocatalyst for the Tandem Hydrolysis and Reduction of p-Nitrophenol Esters. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, v. 38, n. 10, AUG 2021. (19/19551-3, 18/02435-8, 17/20892-4, 15/26308-7, 18/13492-2, 19/09668-0)

MALLAUPOMA CAMARENA, DENISSE ESTHER; ALCANTARA SEKIMOTO MATSUYAMA, LARISSA SATIKO; MARIA-ENGLER, SILVYA STUCHI; CATALANI, LUIZ HENRIQUE. Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing. NANOMATERIALS, v. 10, n. 12, DEC 2020. (11/21442-6, 18/13492-2, 17/04926-6, 19/14527-7)

NOVAES, STEPHANIE DIAS; OLIVEIRA, PEDRO VITORIANO; SIQUEIRA PETRI, DENISE FREITAS. Hydroxypropyl methylcellulose-sugarcane bagasse adsorbents for removal of 17 alpha-ethinylestradiol from aqueous solution and freshwater. Environmental Science and Pollution Research, v. N/A, p. 17-pg., 2022-04-25. (18/13492-2)

DA SILVA, ARUA C.; DA SILVA, RUBENS A.; SOUZA, MARIA J. P. G.; MONTOYA, PAULA M.; BENTINI, RICARDO; AUGUSTO, TATIANA; TORRESI, ROBERTO M.; CATALANI, LUIZ H.; DE TORRESI, SUSANA I. CORDOBA. Electrochemical quartz crystal microbalance with dissipation investigation of fibronectin adsorption dynamics driven by electrical stimulation onto a conducting and partially biodegradable copolymer. BIOINTERPHASES, v. 15, n. 2, MAR 2020. (18/13492-2, 17/08349-3, 15/26308-7, 17/00705-5, 14/09353-6)

DA SILVA SUSANA, ARUA C.; CORDOBA DE TORRESI, I.. Advances in Conducting, Biodegradable and Biocompatible Copolymers for Biomedical Applications. FRONTIERS IN MATERIALS, v. 6, MAY 8 2019. (14/09353-6, 18/13492-2, 17/00705-5, 15/26308-7)

DEZOTTI, RAFAEL S.; FURTADO, LAISE M.; YEE, MARCIO; VALERA, TICIANE S.; BALAJI, KRISHNASAMY; ANDO, ROMULO A.; PETRI, DENISE F. S.. Tuning the Mechanical and Thermal Properties of Hydroxypropyl Methylcellulose Cryogels with the Aid of Surfactants. GELS, v. 7, n. 3, SEP 2021. (18/13492-2)

LEITE, MARINA M.; MARTINS, VITOR L.; VICHI, FLAVIO M.; TORRESI, ROBERTO M.. Electrochemistry of sodium titanate nanotubes as a negative electrode for sodium-ion batteries. Electrochimica Acta, v. 331, JAN 20 2020. (15/26308-7, 18/13492-2, 19/02669-1)

MONTANHEIRO, THAIS LARISSA DO AMARAL; SCHATKOSKI, VANESSA MODELSKI; CAMARENA, DENISSE ESTHER MALLAUPOMA; DE OLIVEIRA, THAIS CARDOSO; DA SILVA, DIEGO MORAIS; VEGIAN, MARIANA RAQUEL DA CRUZ; CATALANI, LUIZ HENRIQUE; KOGA-ITO, CRISTIANE YUMI; THIM, GILMAR PATROCINIO. Evaluating the Cytotoxicity of Functionalized MWCNT and Microbial Biofilm Formation on PHBV Composites. C-JOURNAL OF CARBON RESEARCH, v. 10, n. 2, p. 14-pg., 2024-06-01. (11/21442-6, 17/24873-4, 19/05856-7, 20/12507-6, 18/13492-2)

ROCHA, IGOR; CERQUEIRA, GABRIELLE; PENTEADO, FELIPE VARELLA; DE TORRESI, SUSANA I. CORDOBA. Electrical Stimulation and Conductive Polymers as a Powerful Toolbox for Tailoring Cell Behaviour in vitro. FRONTIERS IN MEDICAL TECHNOLOGY, v. 3, p. 18-pg., 2021-07-29. (18/11162-5, 18/13492-2, 18/21546-5, 15/26308-7, 20/15005-1)

DE BARROS, HELOISE RIBEIRO; DA SILVA, RAFAEL TRIVELLA PACHECO; FERNANDES, RAFAELLA; TORO-MENDOZA, JHOAN; COLUZZA, IVAN; TEMPERINI, MARCIA L. A.; DE TORRESI, SUSANA I. CORDOBA. Unraveling the Nano-Bio Interface Interactions of a Lipase Adsorbed on Gold Nanoparticles under Laser Excitation. Langmuir, v. 40, n. 11, p. 10-pg., 2024-03-07. (17/20892-4, 16/21070-5, 18/13492-2, 18/25422-9, 19/09668-0, 21/00675-4)

ROCHA, BIANCA C.; DOURADO, ISABELA L. A.; SANABRIA, MARIALY N.; KIMURA, NOEMI S. P.; CORDEIRO, PRISCILA H.; CATALANI, LUIZ H.; ANDRADE, LEANDRO H.. Incorporation of renewable carbons via formamide reactivity for the production of novel biobased polymers. GREEN CHEMISTRY, v. 26, n. 12, p. 12-pg., 2024-05-29. (18/12602-9, 19/10762-1, 21/12555-3, 22/15898-1, 18/13492-2)

CALLISAYA, MARLEIDY P.; FUENTES, DAIRON P.; BRAGA, VICTOR H. A.; FINZI-QUINTAO, CRISTIANE M.; OLIVEIRA, PEDRO, V; PETRI, DENISE F. S.. Harnessing carboxymethyl cellulose and Moringa oleifera seed husks for sustainable treatment of a multi-metal real waste. Environmental Research, v. 252, p. 12-pg., 2024-04-25. (18/13492-2)

DELECHIAVE, GIOVANNE; SILVA, MARIANGELA DE OLIVEIRA; DE CASTRO-AMARANTE, MARIA FERNANDA; CAMARENA, DENISSE ESTHER MALLAUPOMA; VENCESLAU-CARVALHO, ALEXIA A.; DE SOUZA FERREIRA, LUIS CARLOS; CATALANI, LUIZ HENRIQUE. Layer-by-Layer Assembly of Polymeric Nanoparticles with Heparin-RBD Complexes as an Adjuvant for SARS-CoV-2 Protein-Based Vaccines. ACS APPLIED NANO MATERIALS, v. 7, n. 4, p. 10-pg., 2024-02-06. (18/14532-8, 21/11971-3, 18/13492-2)

QUEIROZ, LUCAS G.; FAUSTINO, LEANDRO A.; DE OLIVEIRA, PAULO F. M.; POMPEO, MARCELO; DE TORRESI, SUSANA I. CORDOBA. Transformative nanobioplasmonic effects: Toxicological implications of plasmonic silver nanoparticles in aquatic biological models. Science of The Total Environment, v. 954, p. 11-pg., 2024-10-01. (21/00675-4, 22/13146-2, 20/14955-6, 22/04604-7, 18/13492-2)

NUNES, MARIO A. B. S.; FUENTES, DAIRON P.; MESQUITA, JOSE A. F. S.; ROMANO, ROBERTO C. O.; PILEGGI, RAFAEL G.; OLIVEIRA, PEDRO V.; PETRI, DENISE F. S.. Feasibility of sugarcane bagasse/polydopamine as sustainable adsorbents for Cr(VI) with reusability in cement composition. JOURNAL OF HAZARDOUS MATERIALS ADVANCES, v. 12, p. 13-pg., 2023-08-30. (21/07305-8, 18/13492-2)

ALAVARSE, ALEX C.; MIRZAEI, MAHTA; SHAVANDI, AMIN; PETRI, DENISE F. S.. Improved anti-inflammatory properties of xanthan gum hydrogel physically and chemically modified with yeast derived peptide. Biomedical Materials, v. 18, n. 2, p. 13-pg., 2023-03-01. (18/13492-2, 19/22671-0)

FRACHINI, EMILLI C. G.; SELVA, JESSICA S. G.; FALCOSWKI, PAULA C.; SILVA, JEAN B.; CORNEJO, DANIEL R.; BERTOTTI, MAURO; ULRICH, HENNING; PETRI, DENISE F. S.. Caffeine Release from Magneto-Responsive Hydrogels Controlled by External Magnetic Field and Calcium Ions and Its Effect on the Viability of Neuronal Cells. POLYMERS, v. 15, n. 7, p. 22-pg., 2023-04-01. (18/13492-2, 18/07366-4, 18/08782-1)

DA SILVA, DANIEL J.; CATALANI, LUIZ H.. Argon nonthermal plasma etching of poly(L-lactic acid) films: Tunning the local surface degradation and hydrolytic degradation rate. REACTIVE & FUNCTIONAL POLYMERS, v. 200, p. 17-pg., 2024-05-11. (22/16427-2, 18/13492-2)

LIBERATO, MICHELLE S.; CAVALCANTE, NAYARA G. S.; SINDU, P. ABINAYA; RODRIGUES-JESUS, MONICA J.; ZELENOVSKII, PAVEL; CARREIRA, ANA C. O.; BAPTISTA, MAURICIO S.; SOGAYAR, MARI C.; FERREIRA, LUIS C. S.; CATALANI, LUIZ H.. Histidine-based hydrogels via singlet-oxygen photooxidation. SOFT MATTER, v. 17, n. 48, NOV 2021. (18/13492-2, 19/08975-7, 19/10789-7)

ALAVARSE, ALEX CARVALHO; FRACHINI, EMILLI CAROLINE G.; SILVA, JEAN BEZERRA; PEREIRA, RAFAEL DOS SANTOS; ULRICH, HENNING; SIQUEIRA PETRI, DENISE FREITAS. Amino acid decorated xanthan gum coatings: Molecular arrangement and cell adhesion. CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS, v. 4, p. 11-pg., 2022-06-13. (20/01907-3, 18/07366-4, 18/13492-2)

DARIO, BRUNO S.; PEREIRA, RAFAEL; PETRI, DENISE F. S.. Tristyrylphenol based surfactants as efficient dispersants of TiO2 particles in dilute and concentrated dispersions. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 654, p. 11-pg., 2022-12-05. (18/13492-2)

EMILLI C. G. FRACHINI; DENISE F. S. PETRI. Magneto-Responsive Hydrogels: Preparation, Characterization, Biotechnological and Environmental Applications. Journal of the Brazilian Chemical Society, v. 30, n. 10, p. 2010-2028, 2019-10-21. (18/13492-2)

FRACHINI, EMILLI C. G.; PETRI, DENISE F. S.. Magneto-Responsive Hydrogels: Preparation, Characterization, Biotechnological and Environmental Applications. Journal of the Brazilian Chemical Society, v. 30, n. 10, SI, p. 2010-2028, OCT 2019. (18/13492-2)

RIBEIRO DE BARROS, HELOISE; GARCIA, ISABEL; KUTTNER, CHRISTIAN; ZEBALLOS, NICOLL; CAMARGO, PEDRO H. C.; CORDOBA DE TORRESI, SUSANA INES; LOPEZ-GALLEGO, FERNANDO; LIZ-MARZAN, LUIS M.. Mechanistic Insights into the Light-Driven Catalysis of an Immobilized Lipase on Plasmonic Nanomaterials. ACS CATALYSIS, v. 11, n. 1, p. 414-423, JAN 1 2021. (18/13492-2, 19/09668-0, 15/26308-7, 17/20892-4)