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Novel cellular scaffold from a histidine rich recombinant elastin-derived protein for 3D printing

Grant number: 19/10789-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2019
Effective date (End): August 31, 2021
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Luiz Henrique Catalani
Grantee:Michelle da Silva Liberato
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine, AP.TEM


Currently, molecular biology applied to the production of recombinant proteins has proven to be highly effective in biomedicine. In this context, the elastin-like polypeptides (ELPs) are among the most promising stimulation-responsive biopolymers to be expressed genetically. Moreover, the proper expression system depends on the protein produced. In this case, the ELP-40 will be expressed by using the technology of recombinant DNA in lineages of the bacteria Escherichia coli (E. coli). Particularly, it is proposed the development of frameworks produced by 3D printing. For such, we suggest the inclusion of Histidine as a key amino acid in the ELP-40 sequence, targeting the generation of bioactive hydrogels highly elastic and sensitive to photocrosslinking by singlet oxygen. These biomaterials are highly attractive as structural support, aiming the cellular growth, as well as adhesion, proliferation, and differentiation; therefore, they present important uses in the area of regenerative medicine, including its use as inks for 3D printing techniques based on stereolitography. Finally, the scaffolds will be characterized by advanced experimental techniques, so to infer over its structural and morphological properties, besides biological trials for the evaluation of biocompatibility through tests in vitro and in vivo.