Detection of IncN-pST15 one-health plasmid harbouring bla(KPC-2) in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil

Autor(es):Mostrar menos -

Sellera, Fabio P. ^{[1, 2]} ; Fuga, Bruna ^{[3, 4, 2]} ; Fontana, Herrison ^[2] ; Esposito, Fernanda ^{[3, 2]} ; Cardoso, Brenda ^{[3, 4]} ; Konno, Sibele ^[5] ; Berl, Carla ^[5] ; Cappellanes, Mariana H. ^[5] ; Cortez, Marcia ^[5] ; Ikeda, Marcelo ^[5] ; de Souza, Cesar M. ^[1] ; Cerdeira, Louise ^[6] ; Lincopan, Nilton ^{[3, 4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil

^[2] One Hlth Brazilian Resistance Project OneBR, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, Sao Paulo - Brazil

^[5] PetCare Vet Hosp, Sao Paulo - Brazil

^[6] Monash Univ, Dept Infect Dis, Cent Clin Sch, Clayton, Vic - Australia

• Tipo de documento: Artigo Científico
• Fonte: TRANSBOUNDARY AND EMERGING DISEASES; v. 68, n. 6, p. 3083-3088, NOV 2021.
• Citações Web of Science: 3

Resumo

The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of cross-transmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the high-risk international clone ST11/CG258, in a dog with urinary tract infection. Strikingly, the bla(KPC-2) gene was carried by a 54-kb IncN plasmid assignated to ST15, which shared 99.8 and 96.8% pairwise identity with IncN-pST15 plasmids from human and environmental K. pneumoniae strains, respectively; all come from an area with high endemicity of KPC-2. Our findings suggest that IncN-pST15 plasmids conferring carbapenem resistance can play as important a role as clonal transmission of K. pneumoniae, representing another major challenge for One Health. (AU)

• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular