Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia

Autor(es)

Calixto, Giovana Maria Fioramonti ^[1] ; Muniz, Bruno Vilela ^[1] ; Castro, Simone R. ^[2] ; de Araujo, Jaiza Samara Macena ^[1] ; Amorim, Klinger de Souza ^[1] ; Ribeiro, Ligia N. M. ^[3] ; Ferreira, Luiz Eduardo Nunes ^[4] ; de Araujo, Daniele Ribeiro ^[5] ; de Paula, Eneida ^[2] ; Franz-Montan, Michelle ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Biosci, BR-13414903 Piracicaba - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083872 Campinas - Brazil

^[3] Fed Univ Uberlandia UFU, Inst Biotechnol, BR-38405302 Uberlandia, MG - Brazil

^[4] Guarulhos Univ UNG, Lab Inflammat & Immunol, BR-07023070 Guarulhos - Brazil

^[5] Fed Univ ABC UFABC, Human & Nat Sci Ctr, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 13, n. 11 NOV 2021.
• Citações Web of Science: 1

Resumo

Recent advances have been reported for needle-free local anesthesia in maxillary teeth by administering a nasal spray of tetracaine (TTC) and oxymetazoline, without causing pain, fear, and stress. This work aimed to assess whether a TTC-loaded hybrid system could reduce cytotoxicity, promote sustained permeation, and increase the anesthetic efficacy of TTC for safe, effective, painless, and prolonged analgesia of the maxillary teeth in dental procedures. The hybrid system based on TTC (4%) encapsulated in nanostructured lipid carriers (NLC) and incorporated into a thermoreversible hydrogel of poloxamer 407 (TTCNLC-HG4%) displayed desirable rheological, mechanical, and mucoadhesive properties for topical application in the nasal cavity. Compared to control formulations, the use of TTCNLC-HG4% slowed in vitro permeation of the anesthetic across the nasal mucosa, maintained cytotoxicity against neuroblastoma cells, and provided a three-fold increase in analgesia duration, as observed using the tail-flick test in mice. The results obtained here open up perspectives for future clinical evaluation of the thermoreversible hybrid hydrogel, which contains TTC-loaded NLC, with the aim of creating an effective, topical, intranasal, needle-free anesthesia for use in dentistry. (AU)

• Processo FAPESP: 18/25920-9 - Hidrogel termorreversível contendo tetracaína encapsulada em carreadores lipídicos nanoestruturados para administração tópica pela via intranasal
• Beneficiário: Bruno Vilela Muniz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/07245-5 - Lidocaína e epinefrina co-incorporadas em sistemas precursores de cristais líquidos mucoadesivos como estratégia para anestesia tópica na cavidade bucal
• Beneficiário: Giovana Maria Fioramonti Calixto Balian
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Temático