Effects of Aerobic Exercise Training on MyomiRs Expression in Cachectic and Non-Cachectic Cancer Mice

Autor(es)

Gomes, Joao Lucas Penteado ^[1] ; Tobias, Gabriel Cardial ^[1] ; Fernandes, Tiago ^[1] ; Silveira, Andre Casanova ^[1] ; Negrao, Carlos Eduardo ^{[1, 2]} ; Chammas, Roger ^[3] ; Brum, Patricia Chakur ^[1] ; Oliveira, Edilamar Menezes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, BR-05508030 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Med Sch, Heart Inst InCor, BR-05508030 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Inst Canc Estado Sao Paulo, Ctr Invest Translac Oncol, BR-01246000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CANCERS; v. 13, n. 22 NOV 2021.
• Citações Web of Science: 0

Resumo

Simple SummaryMuscle wasting is a symptom of the cancer cachexia closely related to the imbalance between protein synthesis and degradation. MyomiRs are small RNA molecules that do not encode proteins and have the function of regulating protein-coding genes, and in this way, myomiRs can regulate the homeostasis of skeletal muscle cells submitted to physiological or pathological stimulus. Aerobic exercise training (AET) is a nonpharmacological adjuvant treatment to prevent cancer cachexia, improving the patient's quality of life. MyomiRs are modulated by cancer and AET, as well. Thus, we propose to investigate the effects promoted by AET on circulating and skeletal muscle myomiRs in cachectic and non-cachectic cancer mice. Exercise is a promising therapy for cancer-associated muscle wasting, revealing the importance to understand the molecular mechanisms involved to preserve muscle mass.We investigated the effects of AET on myomiRs expression in the skeletal muscle and serum of colon cachectic (CT26) and breast non-cachectic (MMTV-PyMT) cancer mice models. Colon cancer decreased microRNA-486 expression, increasing PTEN in tibialis anterior muscle (TA), decreasing the PI3K/mTOR protein pathway, body and muscle wasting, fibers' cross-sectional area and muscle dysfunction, that were not preserved by AET. In contrast, breast cancer decreased those muscle functions, but were preserved by AET. In circulation, the downregulation of microRNA-486 and -206 in colon cancer, and the downregulation of microRNA-486 and upregulation of microRNA-206 expression in breast cancer might be good cancer serum biomarkers. Since the microRNA-206 is skeletal muscle specific, their expression was increased in the TA, serum and tumor in MMTV, suggesting a communication among these three compartments. The AET prevents these effects on microRNA-206, but not on microRNA-486 in MMTV. In conclusion, cancer induced a downregulation of microRNA-486 expression in TA and serum of CT26 and MMTV mice and these effects were not prevented by AET; however, to MMTV, the trained muscle function was preserved, probably sustained by the downregulation of microRNA-206 expression. Serum microRNA-206 is a potential biomarker for colon (decreased) and breast (increased) cancer to monitor the disease evolution and the effects promoted by the AET. (AU)

• Processo FAPESP: 15/04788-7 - Efeito do treinamento físico aeróbio sobre a expressão de myomiRs circulante e muscular em um modelo experimental de câncer de mama
• Beneficiário: João Lucas Penteado Gomes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/22814-5 - Câncer e coração: novos paradigmas de diagnóstico e tratamento
• Beneficiário: Carlos Eduardo Negrão
• Modalidade de apoio: Auxílio à Pesquisa - Temático