Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status

Autor(es)

Lopes, Alberto Moura Mendes ^[1] ; Martins, Manoela ^[1] ; Goldbeck, Rosana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Bioproc & Metab Engn Lab, Sch Food Engn, Rua Monteiro Lobato 80, Cidade Univ, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: MOLECULAR BIOTECHNOLOGY; v. 63, n. 3, p. 184-199, MAR 2021.
• Citações Web of Science: 1

Resumo

Heterologous expression of the carbohydrate-active enzymes in microorganisms is a promising approach to produce bio-based compounds, such as fuels, nutraceuticals and other value-added products from sustainable lignocellulosic sources. Several microorganisms, including Saccharomyces cerevisiae, Escherichia coli, and the filamentous fungi Aspergillus nidulans, have unique characteristics desirable for a biorefinery production approach like well-known genetic tools, thermotolerance, high fermentative capacity and product tolerance, and high amount of recombinant enzyme secretion. These microbial factories are already stablished in the heterologous production of the carbohydrate-active enzymes to produce, among others, ethanol, xylooligosaccharides and the valuable coniferol. A complete biocatalyst able to heterologous express the CAZymes of glycoside hydrolases, carbohydrate esterases and auxiliary activities families could release these compounds faster, with higher yield and specificity. Recent advances in the synthetic biology tools could expand the number and diversity of enzymes integrated in these microorganisms, and also modify those already integrated. This review outlines the heterologous expression of carbohydrate-active enzymes in microorganisms, as well as recent updates in synthetic biology. (AU)

• Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Regular