Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of Crotalus durissus terrificus (South American Rattlesnake) Venom

Autor(es)

Oliveira, Isadora Caruso Fontana ^[1] ; Yoshida, Edson Hideaki ^[1] ; Dini, Murilo Melo Juste ^[1] ; Paschoal, Ana Beatriz Olivio ^[1] ; Cogo, Jose Carlos ^[2] ; da Cruz-Hofling, Maria Alice ^[3] ; Hyslop, Stephen ^[4] ; Oshima-Franco, Yoko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sorocaba UNISO, Postgrad Program Pharmaceut Sci, Rodovia Raposo Tavares Km 92-5, BR-18023000 Sorocaba, SP - Brazil

^[2] Brazil Univ, Inst Sci & Technol, Bioengn & Biomed Engn Programs, Rua Carolina Fonseca 584-235, BR-08230030 Sao Paulo, SP - Brazil

^[3] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Rua Monteiro Lobato 255, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP - Brazil

^[4] State Univ Campinas UNICAMP, Dept Translat Med, Fac Med Sci, Sect Pharmacol, Rua Tessalia Vieira de Camargo 126, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 13, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Systemic envenomation by Crotalus durissus terrificus (South American rattlesnake) can cause coagulopathy, rabdomyolysis, acute kidney injury, and peripheral neuromuscular blockade, the latter resulting in flaccid paralysis. Previous studies have shown that plant products such as tannic acid and theaflavin can protect against the neuromuscular blockade caused by C. d. terrificus venom in vitro. In this work, we used mouse-isolated phrenic nerve-diaphragm preparations to examine the ability of caffeic acid, chlorogenic acid, and quercetin to protect against C. d. terrificus venom-induced neuromuscular blockade in vitro. In addition, the ability of tannic acid to protect against the systemic effects of severe envenomation was assessed in rats. Preincubation of venom with caffeic acid (0.5 mg/mL), chlorogenic acid (1 mg/mL), or quercetin (0.5 mg/mL) failed to protect against venom (10 mu g/mL)-induced neuromuscular blockade. In rats, venom (6 mg kg(-1), i.p.) caused death in \~{}8 h, which was prevented by preincubation of venom with tannic acid or the administration of antivenom 2 h post-venom, whereas tannic acid given 2 h post-venom prolonged survival (\~{}18.5 h) but did not prevent death. Tannic acid (in preincubation protocols or given 2 h post-venom) had a variable effect on blood creatinine and urea and blood/urine protein levels and prevented venom-induced leukocytosis. Tannic acid attenuated the histological lesions associated with renal damage in a manner similar to antivenom. The protective effect of tannic acid appeared to be mediated by interaction with venom proteins, as assessed by SDS-PAGE. These findings suggest that tannic acid could be a potentially useful ancillary treatment for envenomation by C. d. terrificus. (AU)

• Processo FAPESP: 15/01420-9 - Desenvolvimento e avaliação da eficácia (in vitro e in vivo) de cartucho de hemoperfusor para o tratamento de acidentes ofídicos
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/12599-2 - Tecnologia e protocolo para a técnica de hemoperfusão em modelo animal
• Beneficiário: Murilo Melo Juste Dini
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico