Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from Bothrops Snakes

Autor(es)

Zychar, Bianca C. ^[1] ; Clissa, Patricia B. ^[2] ; Carvalho, Eneas ^[3] ; Alves, Adilson S. ^[4] ; Baldo, Cristiani ^[5] ; Faquim-Mauro, Eliana L. ^[2] ; Goncalves, Luis Roberto C. ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Lab Pathophysiol, BR-05503900 Sao Paulo - Brazil

^[2] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil

^[3] Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05503900 Sao Paulo - Brazil

^[5] Univ Estadual Londrina, Dept Biochem & Biotechnol, BR-86051990 Londrina, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 13, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the role of different SVMPs domains in inducing the expression of adhesion molecules at the microcirculation of the cremaster muscle of mice. We used Jararhagin (Jar)-a PIII SVMP with intense hemorrhagic activity, and Jar-C-a Jar devoid of the catalytic domain, with no hemorrhagic activity, both isolated from B. jararaca venom and BnP-1-a weakly hemorrhagic P1 SVMP from B. neuwiedi venom. Toxins (0.5 mu g) or PBS (100 mu L) were injected into the scrotum of mice, and 2, 4, or 24 h later, the protein and gene expression of CD54 and CD31 in the endothelium, and integrins (CD11a and CD11b), expressed in leukocytes were evaluated. Toxins induced significant increases in CD54, CD11a, and CD11b at the initial time and a time-related increase in CD31 expression. In conclusion, our results suggest that, despite differences in hemorrhagic activities and domain composition of the SVMPs used in this study, they behave similarly to the induction of expression of adhesion molecules that promote leukocyte recruitment. (AU)

• Processo FAPESP: 08/00108-8 - Papel do diferentes domínios de metaloproteases de venenos botrópicos nas alterações da interação leucócito-endotélio da microcirculação do músculo cremaster de camundongos: ação de inibidores de proteases
• Beneficiário: Bianca Cestari Zychar
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/09002-8 - Caracterização estrutural e funcional de uma proteína isolada do plasma de serpentes Bothrops jararaca, relacionada ao cininogênio de alto peso molecular de mamíferos
• Beneficiário: Luis Roberto de Camargo Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular