Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression

Autor(es):Mostrar menos -

Brunetto, Marcio Antonio ^{[1, 2]} ; Ruberti, Bruna ^[3] ; Halfen, Doris Pereira ^[2] ; Caragelasco, Douglas Segalla ^[3] ; Vendramini, Thiago Henrique Annibale ^[1] ; Pedrinelli, Vivian ^[2] ; Macedo, Henrique Tobaro ^[1] ; Jeremias, Juliana Toloi ^[4] ; Pontieri, Cristiana Fonseca Ferreira ^[4] ; Ocampos, Fernanda Maria Marins ^[5] ; Colnago, Luis Alberto ^[5] ; Kogika, Marcia Mery ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Nutr & Prod Dept, Pet Nutrol Res Ctr, BR-13635900 Pirassununga - Brazil

^[2] Univ Sao Paulo, Vet Teaching Hosp, Sch Vet Med & Anim Sci, Vet Nutrol Serv, Cidade Univ, BR-05508270 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Vet Teaching Hosp, Sch Vet Med & Anim Sci, Small Anim Internal Med Serv, Cidade Univ, BR-05508270 Sao Paulo - Brazil

^[4] Nutr Dev Ctr, Grandfood Ind Comercio LTDA Premier Pet R, BR-05508270 Sao Paulo - Brazil

^[5] Embrapa Instrumentacao, R 15 Novembro 1452 Ctr, BR-13560970 Sao Carlos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 11, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 \& PLUSMN; 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 \& PLUSMN; 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by H-1 nuclear magnetic resonance (H-1 NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic profiles between groups and before (CKD-T0) and after renal diet (CKD-T6). Data analysis was performed on SIMCA-P software. Dogs with CKD showed an altered metabolic profile with increased urea, creatinine, creatine, citrate, and lipids. Lactate, branched-chain amino acids (BCAAs), and glutamine were decreased in the CKD group. However, after 6 months of diet, the metabolite profiles of CKD-T0 and CKD-T6 were similar. Metabolomics profile may be useful to evaluate and recognize metabolic dysfunction and progression of CKD, and the diet may have helped maintain and retard the progression of CKD. (AU)

• Processo FAPESP: 18/00504-2 - Investigação metabolômica por RMN de potenciais agroquímicos naturais de Schiekia orinocensis produzidos sob stress biótico e abiótico
• Beneficiário: Fernanda Maria Marins Ocampos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado