Layer-by-Layer Biomimetic Microgels for 3D Cell Culture and Nonviral Gene Delivery

Autor(es)

Carvalho, Bruna G. ^[1] ; Vit, Franciele F. ^[1] ; Carvalho, Hernandes F. ^[2] ; Han, Sang W. ^[3] ; de la Torre, Lucimara G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083852 Campinas - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083865 Campinas - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Biophys, BR-04044010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biomacromolecules; DEC 2021.
• Citações Web of Science: 0

Resumo

Localized release of nucleic acid therapeutics is essential for many biomedical applications, including gene therapy, tissue engineering, and medical implant coatings. We applied the substrate-mediated transfection and layer-by-layer (LbL) technique to achieve an efficient local gene delivery. In the experiments presented herein, we embeded lipoplexes containing plasmid DNA encoding for enhanced green fluorescent protein (pEGFP) within polyelectrolyte alginate-based microgels composed of poly(allylamine hydrochloride) (PAH), chondroitin sulfate (CS), and poly-L-lysine (PLL) with diameters between 70 and 90 mu m. Droplet-based microfluidics was used as the main process to produce the alginate (ALG)-based microgels with discrete size, shape, and low coefficient of variation. The physicochemical and morphological properties of the polyelectrolyte microgels were characterized via optical microscopy, scanning electron microscopy (SEM), and zeta potential analysis. We found that polyelectrolyte microgels provide low cytotoxicity and cell- material interactions (adhesion, spreading, and proliferation). In addition, the microsystem showed the ability to load lipoplexes and a loading efficiency equal to 83%, and it enabled in vitro surface-based transfection of MCF-7 cells. This approach provides a new suitable route for cell adhesion and local gene delivery. (AU)

• Processo FAPESP: 18/18523-3 - Síntese de micropartículas poliméricas via processo microfluídico de gotas para a liberação sustentada de vetores não virais aplicados em terapia gênica
• Beneficiário: Bruna Gregatti de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/20206-8 - Modulação de monócitos, macrófagos e pericitos pelos genes dos fatores estimuladores de colônia para tratamento de isquemia de membros em modelo murino
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/19537-8 - Microfluídica como plataforma tecnológica para nano & biotecnologia
• Beneficiário: Lucimara Gaziola de la Torre
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/20341-8 - Síntese de micropartículas poliméricas via processo microfluídico de gotas para a liberação sustentada de vetores não virais aplicados em terapia gênica
• Beneficiário: Bruna Gregatti de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Mestrado