A Naked Eye-Invisible Ratiometric Fluorescent Microneedle Tattoo for Real-Time Monitoring of Inflammatory Skin Conditions

Autor(es)

Babity, Samuel ^[1] ; Couture, Frederic ^{[2, 3, 4]} ; Campos, Estefania V. R. ^{[5, 6]} ; Hedtrich, Sarah ^[6] ; Hagen, Raphael ^[7] ; Fehr, Daniel ^[7] ; Bonmarin, Mathias ^[7] ; Brambilla, Davide ^[1]

Afiliação do(s) autor(es)

^[1] Univ Montreal, Fac Pharm, CP 6128, Succursale Ctr ville, Montreal, PQ H3C 3J7 - Canada

^[2] TransBIOTech, 201 Monseigneur Bourget Levis, Quebec City, PQ G6V 6Z9 - Canada

^[3] Univ Laval, Nutraceuticals & Funct Foods Inst INAF, Quebec City, PQ G1K 7P4 - Canada

^[4] Ctr Integre Sante & Serv Sociaux Chaudiere Appala, Quebec City, PQ - Canada

^[5] Fed Univ ABC, Human & Nat Sci Ctr, BR-09210580 Santo Andre, SP - Brazil

^[6] Univ British Columbia, Fac Pharmaceut Sci, 2405 Wesbrook Mall, Vancouver, BC V6T 1Z3 - Canada

^[7] Zurich Univ Appl Sci, Sch Engn, Technikumstr 9, CH-8400 Winterthur - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: ADVANCED HEALTHCARE MATERIALS; DEC 2021.
• Citações Web of Science: 0

Resumo

The field of portable healthcare monitoring devices has an urgent need for the development of real-time, noninvasive sensing and detection methods for various physiological analytes. Currently, transdermal sensing techniques are severely limited in scope (i.e., measurement of heart rate or sweat composition), or else tend to be invasive, often needing to be performed in a clinical setting. This study proposes a minimally invasive alternative strategy, consisting of using dissolving polymeric microneedles to deliver naked eye-invisible functional fluorescent ratiometric microneedle tattoos directly to the skin for real-time monitoring and quantification of physiological and pathological parameters. Reactive oxygen species are overexpressed in the skin in association with various pathological conditions. Here, one demonstrates for the first time the microneedle-based delivery to the skin of active fluorescent sensors in the form of an invisible, ratiometric microneedle tattoo capable of sensing reactive oxygen species in a reconstructed human-based skin disease model, as well as an in vivo model of UV-induced dermal inflammation. One also elaborates a universal ratiometric quantification concept coupled with a custom-built, multiwavelength portable fluorescence detection system. Fully realized, this approach presents an opportunity for the minimally invasive monitoring of a broad range of physiological parameters through the skin. (AU)

• Processo FAPESP: 17/24402-1 - SISTEMAS NANOESTRUTURADOS SENSÍVEIS A pH E TEMPERATURA COMO CARREADORES DE FÁRMACOS ATRAVÉS DA PELE
• Beneficiário: Estefânia Vangelie Ramos Campos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/05100-0 - Avaliação da atividade biológica das formulações tópicas de budesonida em modelos de pele inflamatória
• Beneficiário: Estefânia Vangelie Ramos Campos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado