Cervicovaginal levels of human beta defensins during bacterial vaginosis

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Noda-Nicolau, Nathalia Mayumi ^[1] ; Silva, Mariana de Castro ^[1] ; Cosi Bento, Giovana Fernanda ^[1] ; Baptista Ferreira, Jeniffer Sena ^[1] ; Novak, Juliano ^[1] ; Pessoa Morales, Julia Andrade ^[1] ; Tronco, Julia Abbade ^[1] ; Bolpetti, Aline Nascimento ^[1] ; Silva Pinto, Gabriel Vitor ^[1] ; Polettini, Jossimara ^[2] ; Marconi, Camila ^{[1, 3]} ; da Silva, Marcia Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Fed Fronteira Sul UFFS, Med Sch, Grad Program Biomed Sci, Passo Fundo, RS - Brazil

^[3] Univ Fed Parana UFPR, Dept Basic Pathol, Setor Ciencias Biol, Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 12 DEC 2 2021.
• Citações Web of Science: 0

Resumo

Aims To compare the cervicovaginal levels of human beta defensin (hBD)-1, 2 and 3 of women according to the status of Nugent-defined bacterial vaginosis (BV). Methods A total of 634 women of reproductive age were included in the study. Participants were equally distributed in two groups: according to the classification of vaginal smears according to Nugent criteria in normal (scores 0 to 3) and BV (scores >= 7). Cervicovaginal fluid samples were used for measurements of hBDs1, 2 and 3 levels by enzyme-linked immunosorbent assay (ELISA). Levels of each hBD were compared between the two study groups using Mann-Whitney test, with p-value <0.05 considered as significant. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for sociodemographic variables and hBD1-3 levels associated with BV a multivariable analysis. Correlation between Nugent score and measured levels of hBDs1-3 were calculated using Spearman's test. Results Cervicovaginal fluids from women with BV showed lower levels of hBD-1 {[}median 2,400.00 pg/mL (0-27,800.00); p<0.0001], hBD-2 {[}5,600.00 pg/mL (0-45,800.00); p<0.0001] and hBD-3 {[}1,600.00 pg/mL (0-81,700.00); p = 0.012] when compared to optimal microbiota {[}hBD-1: {[}median 3,400.00 pg/mL (0-35,600.00), hBD-2: 12,300.00 pg/mL (0-95,300.00) and hBD-3: 3,000.00 pg/mL (0-64,300.00), respectively]. Multivariable analysis showed that lower levels of hBD-1 (OR: 2.05; 95% CI: 1.46-2.87), hBD-2 (OR: 1.85; 95% CI: 1.32-2.60) and hBD-3 (OR: 1.90; 95% CI: 1.37-2.64) were independently associated BV. Significant negative correlations were observed between Nugent scores and cervicovaginal levels of hBD-1 (Spearman's rho = -0.2118; p = 0.0001) and hBD-2 ({*}Spearman's rho = -0.2117; p = 0.0001). Conclusions Bacterial vaginosis is associated with lower cervicovaginal levels of hBDs1-3 in reproductive-aged women. (AU)

• Processo FAPESP: 12/16800-3 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular