A sugarcane cystatin (CaneCPI-5) alters microcosm biofilm formation and reduces dental caries

Autor(es):Mostrar menos -

Araujo, Tamara Teodoro ^[1] ; Camiloti, Gabriel Domingues ^[1] ; Valle, Aline Dionizio ^[1] ; Silva, Natara Dias Gomes ^[1] ; Souza, Beatriz Martines ^[1] ; Carvalho, Thamyris de Souza ^[1] ; Camara, Joao Victor Frazao ^[1] ; Shibao, Priscila Yumi Tanaka ^[2] ; Henrique-Silva, Flavio ^[2] ; Cruvinel, Thiago ^[1] ; Magalhaes, Ana Carolina ^[1] ; Buzalaf, Marilia Afonso Rabelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolut, Lab Mol Biol, Sao Carlos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 37, n. 1, p. 109-116, JAN 28 2021.
• Citações Web of Science: 2

Resumo

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml(-1) CaneCPI-5, 0.1 mg ml(-1) CaneCPI-5 or 0.5 mg ml(-1) CaneCPI-5. They were incubated with inoculum (human saliva + McBain's saliva) for the first 8 h. From then until the end of the experiment, the enamel was exposed to McBain saliva with sucrose and, once a day, for 5 days, they were treated with the solutions. At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was also measured. All concentrations of CaneCPI-5 and CHX significantly reduced the activity of biofilms compared with PBS. For viable plate counts, all treatments similarly reduced the lactobacilli and total streptococci; for the mutans streptococci, 0.05 mg ml(-1) CaneCPI-5 performed better than CHX. All CaneCPI-5 concentrations significantly reduced the integrated mineral loss. This study represents the first step regarding the use of CaneCPI-5 within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries. (AU)

• Processo FAPESP: 19/26070-1 - Modulação da película adquirida do esmalte e do biofilme para o controle da perda mineral dentária: desvendando mecanismos e possibilitando terapias
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/08032-5 - Engenharia de película adquirida para proteção contra cárie e erosão dentária: efeito da cistatina, estaterina e hemoglobina no perfil proteômico da película adquirida, no biofilme bacteriano e no esmalte dentário
• Beneficiário: Tamara Teodoro Araujo
• Modalidade de apoio: Bolsas no Brasil - Doutorado