| Texto completo | |
| Autor(es): |
Manzato, Veronica Moraes
[1]
;
Soares Torquato, Ricardo Jose
[1]
;
Alves Lemos, Francisco Jose
[2, 3]
;
Nishiduka, Erika
[1]
;
Tashima, Alexandre Keiji
[1]
;
Tanaka, Aparecida Sadae
[3, 1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Bioquim, Escola Paulista Med, Rua 3 Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Estadual Norte Fluminense, Lab Biotecnol, Rio De Janeiro, RJ - Brazil
[3] Inst Nacl Ciencia & Tecnol Entomol Mol INCT Em, Rio de Janeiro, RJ - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biochemical and Biophysical Research Communications; v. 590, p. 139-144, JAN 29 2022. |
| Citações Web of Science: | 0 |
| Resumo | |
In Brazil, the major vector of arboviruses is Aedes aegypti, which can transmit several alpha and flaviviruses. In this work, a pacifastin protease inhibitor library was constructed and used to select mutants for Ae. aegypti larvae digestive enzymes. The library contained a total of 3.25 x 10(5) cfu with random mutations in the reactive site (P2-P2'). The most successfully selected mutant, TiPI6, a versatile inhibitor, was able to inhibit all three Ae. aegypti larvae proteolytic activities, trypsin-like, chymotrypsin-like and elastase-like activities, with IC50 values of 0.212 nM, 0.107 nM and 0.109 nM, respectively. In conclusion, the TiPI mutated phage display library was shown to be a useful tool for the selection of an inhibitor of proteolytic activities combined in a mix. TiPI6 is capable of controlling all three digestive enzyme activities present in the larval midgut extract. To our knowledge, this is the first time that one inhibitor containing a Gln at the P1 position showed inhibitory activity against trypsin, chymotrypsin, and elastase-like activities. TiPI6 can be a candidate for further larvicidal studies. (C) 2021 Published by Elsevier Inc. (AU) | |
| Processo FAPESP: | 12/03657-8 - Inibidores e proteases de ectoparasitas: relação de estrutura-função e identificação do papel dessas moléculas na interação de vetores de doenças e seus agentes etiológicos |
| Beneficiário: | Aparecida Sadae Tanaka |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/03779-5 - Uso de phage display como ferramenta no diagnóstico e controle de doenças transmitidas por vetores hematófagos |
| Beneficiário: | Aparecida Sadae Tanaka |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |