Segment fusion chip calorimetry: a new method for the investigation of fast reactions

Autor(es)

Lerchner, J. ^[1] ; Sartori, M. R. ^[2] ; Volpe, P. O. ^[3] ; Forster, S. ^[4] ; Mazik, M. ^[4] ; Vercesi, A. E. ^[2] ; Mertens, F. ^[1]

Afiliação do(s) autor(es)

^[1] Tech Univ Bergakad Freiberg, Inst Phys Chem, Leipziger Str 29, D-09599 Freiberg - Germany

^[2] Univ Estadual Campinas, Dept Clin Pathol, UNICAMP, BR-13083877 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[4] Tech Univ Bergakad Freiberg, Inst Organ Chem, Leipziger Str 29, D-09599 Freiberg - Germany

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY; v. 147, n. 3, p. 2253-2263, FEB 2022.
• Citações Web of Science: 2

Resumo

The development of the segment-flow technology has considerably extended the application range of miniaturized calorimeters. The transport of samples in aqueous mu L segments by a water-immiscible carrier liquid enables the investigation of aggregated, complex biological samples in flow-through. The protection from contaminations of the fluidics of the calorimeter by the carrier liquid is an excellent precondition for the design of efficient automatic experiment protocols. In this paper, a new operation mode of this technique is presented, which allows the measurement of fast transient heat-dissipating processes using a chip calorimeter. Heat dissipation is initiated by the merging of separate mu L-sample-segments arriving successively at the measuring chamber. The potential of the segment fusion technique was demonstrated here using two different applications. First, we showed the use of this method for an automatic screening of the strength of receptor-substrate interactions by the measurement of the heat of the adduct formation of carbohydrate octyl ss-d-glucopyranoside and three different receptor molecules. Next, we used the method for the determination of transient heat effects in the seconds range allowing the investigation of metabolic processes in Trypanosoma cruzi cells induced by Ca2+ ions. (AU)

• Processo FAPESP: 17/17728-8 - Função e disfunção mitocondrial: implicações para o envelhecimento e doenças associadas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/05487-6 - Funções mitocondriais e marcadores moleculares associados ao envelhecimento em uma espécie longeva, a tartaruga terrestre Chelonoidis carbonaria (Jabuti piranga)
• Beneficiário: Marina Rincon Sartori
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/19976-1 - Investigações do metabolismo energético do Trypanosoma cruzi com o chip-calorímetro
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional