| Full text | |
| Author(s): |
Lerchner, J.
[1]
;
Sartori, M. R.
[2]
;
Volpe, P. O.
[3]
;
Forster, S.
[4]
;
Mazik, M.
[4]
;
Vercesi, A. E.
[2]
;
Mertens, F.
[1]
Total Authors: 7
|
| Affiliation: | [1] Tech Univ Bergakad Freiberg, Inst Phys Chem, Leipziger Str 29, D-09599 Freiberg - Germany
[2] Univ Estadual Campinas, Dept Clin Pathol, UNICAMP, BR-13083877 Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil
[4] Tech Univ Bergakad Freiberg, Inst Organ Chem, Leipziger Str 29, D-09599 Freiberg - Germany
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY; v. 147, n. 3, p. 2253-2263, FEB 2022. |
| Web of Science Citations: | 2 |
| Abstract | |
The development of the segment-flow technology has considerably extended the application range of miniaturized calorimeters. The transport of samples in aqueous mu L segments by a water-immiscible carrier liquid enables the investigation of aggregated, complex biological samples in flow-through. The protection from contaminations of the fluidics of the calorimeter by the carrier liquid is an excellent precondition for the design of efficient automatic experiment protocols. In this paper, a new operation mode of this technique is presented, which allows the measurement of fast transient heat-dissipating processes using a chip calorimeter. Heat dissipation is initiated by the merging of separate mu L-sample-segments arriving successively at the measuring chamber. The potential of the segment fusion technique was demonstrated here using two different applications. First, we showed the use of this method for an automatic screening of the strength of receptor-substrate interactions by the measurement of the heat of the adduct formation of carbohydrate octyl ss-d-glucopyranoside and three different receptor molecules. Next, we used the method for the determination of transient heat effects in the seconds range allowing the investigation of metabolic processes in Trypanosoma cruzi cells induced by Ca2+ ions. (AU) | |
| FAPESP's process: | 17/17728-8 - Mitochondrial function and dysfunction: implications for aging and associated diseases |
| Grantee: | Aníbal Eugênio Vercesi |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/05487-6 - Mitochondrial function and molecular markers associated with aging in the long-lived red-footed tortoise Chelonoidis carbonaria |
| Grantee: | Marina Rincon Sartori |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/19976-1 - Chip-calorimetric investigations on the energy metabolism of Trypanosoma cruzi |
| Grantee: | Aníbal Eugênio Vercesi |
| Support Opportunities: | Research Grants - Visiting Researcher Grant - International |