reventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertensio

Autor(es):Mostrar menos -

Mariano, Thaoan Bruno ^[1] ; de Souza Castilho, Anthony Cesar ^[1] ; Dias de Almeida Sabela, Ana Karenina ^[1] ; de Oliveira, Andre Casanova ^[1] ; Cury, Sarah Santiloni ^[2] ; Aguiar, Andreo Fernando ^[3] ; Dias, Raisa de Jesus Dutra ^[4] ; Cicogna, Antonio Carlos ^[5] ; Okoshi, Katashi ^[5] ; Justulin Junior, Luis Antonio ^[2] ; Carvalho, Robson Francisco ^[2] ; Pacagnelli, Francis Lopes ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Western Sao Paulo UNOESTE, Postgrad Program Anim Sci, Presidente Prudente, SP - Brazil

^[2] UNESP, Dept Struct & Funct Biol, Inst Biosci, Botucatu, SP - Brazil

^[3] Northern Univ Parana, Postgrad Program Phys Exercise Hlth Promot, Londrina, Parana - Brazil

^[4] Univ Western Sao Paulo UNOESTE, Dept Physiotherapy, Presidente Prudente, SP - Brazil

^[5] UNESP, Dept Internal Med, Botucatu Med Sch, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 9 SEP 8 2021.
• Citações Web of Science: 0

Resumo

To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH. (AU)

• Processo FAPESP: 18/12526-0 - Avaliação da expressão gênica das miosinas cardíacas em ratos com hipertensão pulmonar submetidos ao treinamento preventivo
• Beneficiário: Raisa de Jesus Dutra Dias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/24317-7 - Análise das vias moleculares envolvendo o colágeno no ventrículo direito de ratos com hipertensão pulmonar submetidos ao treinamento físico
• Beneficiário: Lauren Chrys Soato Marin Schaffer
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico