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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

reventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertensio

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Mariano, Thaoan Bruno [1] ; de Souza Castilho, Anthony Cesar [1] ; Dias de Almeida Sabela, Ana Karenina [1] ; de Oliveira, Andre Casanova [1] ; Cury, Sarah Santiloni [2] ; Aguiar, Andreo Fernando [3] ; Dias, Raisa de Jesus Dutra [4] ; Cicogna, Antonio Carlos [5] ; Okoshi, Katashi [5] ; Justulin Junior, Luis Antonio [2] ; Carvalho, Robson Francisco [2] ; Pacagnelli, Francis Lopes [4, 1]
Total Authors: 12
[1] Univ Western Sao Paulo UNOESTE, Postgrad Program Anim Sci, Presidente Prudente, SP - Brazil
[2] UNESP, Dept Struct & Funct Biol, Inst Biosci, Botucatu, SP - Brazil
[3] Northern Univ Parana, Postgrad Program Phys Exercise Hlth Promot, Londrina, Parana - Brazil
[4] Univ Western Sao Paulo UNOESTE, Dept Physiotherapy, Presidente Prudente, SP - Brazil
[5] UNESP, Dept Internal Med, Botucatu Med Sch, Botucatu, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 16, n. 9 SEP 8 2021.
Web of Science Citations: 0

To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH. (AU)

FAPESP's process: 18/12526-0 - Evaluation of gene expression of cardiac myosins in rats with pulmonary hypertension submitted to preventive training
Grantee:Raisa de Jesus Dutra Dias
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/24317-7 - Analysis of the molecular pathways involving the collagen in the right ventricle of rats with pulmonary hypertension submitted to physical training
Grantee:Lauren Chrys Soato Marin Schaffer
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training